FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431132
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAATAATGATTAACTAGA32630.7568447714389096No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC21100.48940927604538753No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20180.4680701038197118No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG17290.4010372693281872No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14920.34606570609465315No Hit
CCCTTATCCAATGGCAAATATTTGGTTACATTTGGATAAAACCTAGCAGG14900.34560181104626886No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG12920.2996762012562278No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12240.2839037696111632No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11410.2646521251032167No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG10590.24563242811946223No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT10430.24192126773238823No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTATT10070.23357115686147167No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10010.2321794717163189No Hit
CATTTAAACCCTAACAAAACAAAACGATGGGGTTACTCTTTACATTTCAT9930.23032389152278188No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTTACATTTGGATAAAAC9900.22962804895020553No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCAAA9820.2277724687566685No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC9580.22220572817605744No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTACTTTGGTTTCACATTTCTT9360.21710288264383065No Hit
CATTTACACCAAGACATTATCAAAAAGTGTGAACAATTTGTAGGCCCACT9030.20944861434549047No Hit
CCTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCCTGTAT8560.19854708070846053No Hit
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAATAATGATTAACTAGA8480.19669150051492348No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACCGAGT8450.1959956579423471No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8440.195763710418155No Hit
CACTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGG8030.1862538619262778No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTTACATT7870.1825427015392038No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC7870.1825427015392038No Hit
GTCCCGTGCTGGTTGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7840.1818468589666274No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7790.18068712134566675No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC7770.18022322629728252No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG7760.17999127877309037No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7660.17767180353116913No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7480.17349674809571083No Hit
CATTTACACCAAGACATTATCAAAAATTGTGAACAATTTGTAGGCCCACT7380.17117727285378956No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7240.1679300075150998No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7150.16584247979737063No Hit
GTGTAAATAATGTCTGGTTTGGAAATAATGATTAACTAGATGTTCTGGAT7120.16514663722479425No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7110.16491468970060214No Hit
GTCTAGTTTGGAAATAATGATTAACTAGATGTTCTGGATAATAAGGTTTA7090.1644507946522179No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7080.16421884712802576No Hit
GTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA6860.15911600159579897No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTG6800.15772431645064622No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT6800.15772431645064622No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6790.15749236892645407No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6740.15633263130549344No Hit
CCCACTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTA6700.15540484120872494No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT6640.1540131560635722No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6620.15354926101518793No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG6590.15285341844261155No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6380.14798252043457688No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6280.1456630451926556No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCCGC6190.14357551747492647No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6140.14241577985396583No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTTTGGGTCACCATATT6120.1419518848055816No Hit
ATCCCAGAGTGAGGGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6100.14148798975719734No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA5990.13893656699108392No Hit
GAATACAGGCATACAAGGGCATTAAGGCAGGATAACCACATTGTGTAAAA5970.1384726719426997No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT5940.13777682937012328No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTGTTCCTGTC5910.13708098679754693No Hit
TTCTTCCACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5870.1361531967007784No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC5790.1342976165072414No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTT5770.13383372145885716No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5720.13267398383789653No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5620.13035450859597525No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5610.1301225610717831No Hit
GCTTTATTCTTCCACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5530.12826698087824612No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5480.12710724325728548No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5450.12641140068470907No Hit
GTCTATAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5440.12617945316051699No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5420.1257155581121327No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5380.1247877680153642No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5330.12362803039440357No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5300.1229321878218272No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACGTACCCCATGAA5300.1229321878218272No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTTTGGGTCACCATA5290.12270024029763507No Hit
CTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5260.12200439772505868No Hit
ATCAAAAAGTGTGAACAATTTGTAGGCCCACTAACAGTCAATGAAAAAAG5210.12084466010409804No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACAACC5160.1196849224831374No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5160.1196849224831374No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5130.11898907991056103No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5130.11898907991056103No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA5120.1187571323863689No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5100.11829323733798466No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5070.11759739476540827No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5060.11736544724121616No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5050.11713349971702403No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTACTTTGGTTT5020.11643765714444763No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAAT5020.11643765714444763No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCTT5010.11620570962025552No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTAACCAAATATTTGCCATTGG4970.115277919523487No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4950.11481402447510276No Hit
GAGTAACCCCATCGTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4950.11481402447510276No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4920.11411818190252636No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4920.11411818190252636No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTATTTCCAAACC4920.11411818190252636No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4910.11388623437833426No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4890.11342233932995No Hit
GGCATACAAGGGCATTAAGGCAGGATAACCACATTGTGTAAAAGGAGCAG4820.1117987066606051No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4810.11156675913641298No Hit
ATTTGTAGGCCCACTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTA4790.11110286408802872No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4760.11040702151545236No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC4740.10994312646706808No Hit
CTCTTTACATTTCATGGGGTACGTCATTGGATGTTATGGGTCATTGCCAC4740.10994312646706808No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCACTGCATG4740.10994312646706808No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4720.10947923141868385No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4720.10947923141868385No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4710.10924728389449172No Hit
ATGTGGTTATCCTGCCTTAATGCCCTTGTATGCCTGTATTCAATCTAAGC4690.10878338884610746No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4680.10855144132191533No Hit
TCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATC4650.10785559874933895No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4640.10762365122514682No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4640.10762365122514682No Hit
AGATCACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA4620.10715975617676257No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4620.10715975617676257No Hit
CACCAAGACATTATCAAAAAGTGTGAACAATTTGTAGGCCCACTAACAGT4610.10692780865257044No Hit
GGCATAATCAATTGCAGTCTTCTTTTTTCATTGACTGTTAGTGGGCCTAC4610.10692780865257044No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGAATGT4550.10553612350741769No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTACT4540.10530417598322556No Hit
GACGTACCCCATGAAATGTAAAGAGTAACCCCATCGTTTTGTTTTGTTAG4530.10507222845903341No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4510.10460833341064918No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4480.10391249083807279No Hit
AAAATTGTGAACAATTTGTAGGCCCACTAACAGTCAATGAAAAAAGAAGA4470.10368054331388067No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG4450.10321664826549641No Hit
GGGTTACTCTTTACATTTCATGGGGTACGTCATTGGATGTTATGGGTCAT4410.1022888581687279No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4410.1022888581687279No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4390.10182496312034364No Hit
GGGAAATACAGGCCCCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAAGT4360.10112912054776728No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4360.10112912054776728No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4350.10089717302357515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)