FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347693
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA17640.5073441225448888No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12580.3618134388670465No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG10940.31464539119280516No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9830.28272067599865397No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA9770.28099501571788904No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9320.2680525636121521No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9080.26114992248909236No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8260.23756589865197172No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7800.22433583649944058No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7720.22203495612508733No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAAATTTCAT7290.2096677241129387No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7230.20794206383217378No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7220.20765445378537964No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7210.2073668437385855No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7120.2047783533174381No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC6820.19615005191361343No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6680.19212351125849528No Hit
CTACTGTACCTGTCTTTAACTCTCATTGGAAAACACCCTCTTTTCCTAAT6430.18493326008864142No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG6310.18148193952711156No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6110.17572973859122848No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5990.17227841802969862No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC5950.17112797784252198No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT5690.16365011662587398No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5660.1627872864854915No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5600.16106162620472658No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5580.1604864061111383No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC5560.15991118601754997No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5290.1521457147541078No Hit
CAATAGGCCTGTGAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGT5260.15128288461372533No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5230.15042005447334286No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5190.14926961428616622No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT5140.14783156405219547No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5040.14495546358425393No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT4910.14121653297592993No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4900.14092892292913575No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA4790.13776521241440007No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4770.13718999232081175No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4690.13488911194645853No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4580.13172540143172282No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4520.1299997411509579No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGAGTTAAAGA4430.1274112507298105No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC4420.12712364068301632No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4380.1259732004958397No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4340.1248227603086631No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4250.12223426988751572No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA4240.12194665984072155No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4240.12194665984072155No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4210.1210838297003391No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4160.11964577946636831No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4140.11907055937278001No Hit
TTCTTCTACTGTACCTGTCTTTAACTCTCATTGGAAAACACCCTCTTTTC4130.11878294932598586No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4080.11734489909201508No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4060.11676967899842676No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4000.11504401871766183No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC3880.11159269815613199No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3870.11130508810933784No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT3870.11130508810933784No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA3840.11044225796895538No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3830.11015464792216122No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3820.10986703787536707No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG3800.10929181778177877No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3790.10900420773498459No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3770.10842898764139629No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3730.10727854745421966No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3700.10641571731383721No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA3670.10555288717345473No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC3670.10555288717345473No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3670.10555288717345473No Hit
TGGATACGATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGA3660.1052652771266606No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA3650.10497766707986643No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT3630.10440244698627812No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3570.1026767867055132No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG3560.10238917665871905No Hit
GTCTTTAACTCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3550.10210156661192489No Hit
ACATAGAGGTTCCTTGTGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3510.10095112642474827No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3510.10095112642474827No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3490.10037590633115996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)