FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347693
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16990.48864946950326865No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12550.360950608726664No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10120.29106136735568444No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA10050.2890480970281254No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG10020.28818526688774293No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8850.25453489141282687No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8450.24303048954106066No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAAATTTCAT8100.23296413790326523No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8050.23152608766929447No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8040.2312384776225003No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7740.22261017621867568No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7600.21858363556355753No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7240.20822967387896796No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7180.206504013598203No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6990.20103942270911407No Hit
CTACTGTACCTGTCTTTAACTCTCATTGGAAAACACCCTCTTTTCCTAAT6560.18867219069696542No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG6260.1800438892931408No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC6070.17457929840405184No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6010.1728536381232869No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5920.17026514770213955No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5850.16825187737458044No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5830.16767665728099213No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT5660.1627872864854915No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5530.15904835587716748No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5530.15904835587716748No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5450.15674747550281426No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT5410.15559703531563765No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5220.15013244442654872No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5090.1463935138182247No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC4960.14265458320990068No Hit
CAATAGGCCTGTGAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGT4940.1420793631163124No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4900.14092892292913575No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4750.13661477222722346No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA4720.13575194208684097No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4660.13402628180607604No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4630.1331634516656936No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4550.13086257129134035No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4500.12942452105736957No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGAGTTAAAGA4490.12913691101057542No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4390.12626081054263388No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4390.12626081054263388No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4380.1259732004958397No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4360.12539798040225142No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC4330.12453515026186895No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4320.12424754021507478No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4310.12395993016828064No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4220.12137143974713324No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT4210.1210838297003391No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4160.11964577946636831No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4140.11907055937278001No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4060.11676967899842676No Hit
TTCTTCTACTGTACCTGTCTTTAACTCTCATTGGAAAACACCCTCTTTTC3940.1133183584368969No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT3930.11303074839010277No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC3840.11044225796895538No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT3840.11044225796895538No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3830.11015464792216122No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3830.11015464792216122No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3820.10986703787536707No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG3760.10814137759460214No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA3720.10699093740742553No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3610.10382722689268982No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3520.10123873647154243No Hit
GTCTTTAACTCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3520.10123873647154243No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA3510.10095112642474827No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA3500.10066351637795412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)