FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422766
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24610.5821187134253937No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA22490.531972769806465No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC14940.3533869800315068No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT12020.28431803882052953No Hit
GTGTAAAAGGGGCAGCAAACCCTAAAAGACCTACAATACGTTGACAGACT11430.27036232809639377No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10910.2580623796615622No Hit
ATGTTATTGGATGTTATGGGTCCTTACCACAAGATCACATCATACAGAAA10590.25049318062474274No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10200.2412682192986191No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8930.211227960621242No Hit
TTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGT8800.20815297351253412No Hit
GCTTCGACTGAATACATGCATATAAAGGCATTAAAGCAGGATAACCACAT8780.20767989857273292No Hit
GGATAAGGGTATCAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8580.20294914917472076No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCTAAAAGACCTACAAT8570.20271261170482016No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8390.19845493724660923No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA8170.1932511129087959No Hit
CCCTAAAAGACCTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT8070.19088573820978982No Hit
CATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATT7940.18781075110108192No Hit
CAATAACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG7370.17432811531674733No Hit
TATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAA7270.17196274061774125No Hit
GTTTCCTAAAGCATTCTTTGATTTTCTGTATGATGTGATCTTGTGGTAAG7070.16723199121972912No Hit
TATACAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAA6900.16321085423141882No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6890.1629743167615182No Hit
GTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT6840.16179162941201514No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6840.16179162941201514No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6670.15777049242370483No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6530.15445896784509636No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6500.1537493554353945No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6450.15256666808589148No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6190.14641669386847572No Hit
CCCTTATCCAATGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGG6040.1428686318199666No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5970.14121286953066237No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5970.14121286953066237No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAGCATTCTTTGATTTTCTGT5880.1390840323015569No Hit
CATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCA5850.13837441989185506No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5740.13577250772294838No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAACCCTAAAAGACCTACAATACG5660.13388020796374353No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5630.1331705955540417No Hit
GAATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5380.12725715880652655No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAGTCTCCTCC5380.12725715880652655No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5290.12512832157742107No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5230.12370909675801743No Hit
CTTTGGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5220.12347255928811683No Hit
GGATAATAAGGTTTGATACCCTTATCCAATGGCAAATATTTAGTAACATT5220.12347255928811683No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT5200.12299948434831562No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5110.12087064711921015No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5040.11921488482990591No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5000.11826873495030348No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4990.11803219748040288No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4940.11684951013089984No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4920.11637643519109864No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT4890.1156668227813968No Hit
ACACTAATGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCT4860.11495721037169497No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC4860.11495721037169497No Hit
CTCTTTACATTTCATGGGCTATGTTATTGGATGTTATGGGTCCTTACCAC4850.11472067290179437No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACTGT4770.11282837314258952No Hit
TCCTAATACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGT4700.11117261085328528No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4550.10762454880477616No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4540.10738801133487556No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATT4520.10691493639507434No Hit
GCACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4420.10454956169606827No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4350.10289379940676402No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTT4320.1021841869970622No Hit
ACTCTTTACATTTCATGGGCTATGTTATTGGATGTTATGGGTCCTTACCA4270.10100149964755918No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA4260.10076496217765857No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC4240.10029188723785734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)