FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580837
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA47710.8214008405111933No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33380.5746879072786341No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA20770.3575874126476103No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT18320.315406904174493No Hit
GGATAAGGGTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACT17260.2971573780595933No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT16750.28837694568355665No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT13810.23776033551581596No Hit
ACTACGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG13390.23052939120613872No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC13250.22811907643624632No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACGTAGCGCC12530.21572317190537105No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12140.2090087236178136No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTCCAAACC12070.2078035662328674No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12050.2074592355514542No Hit
TAATAACTCTGTCTACTTGGGTGGGTGGTAATTTGGAAGATCCAACATCT11880.20453242475944197No Hit
ATATAAGACCTTGGGCAAGACTTGATGGGCGTTCACGGTGGTTTCCATGC11880.20453242475944197No Hit
GTATACATGCATACACGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA11870.20436025941873537No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11800.2031551020337892No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11430.19678498442764494No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT10690.18404474921535646No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT10580.18215093046758385No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC10530.18129010376405083No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT10510.18094577308263765No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9500.16355707367127092No Hit
CATGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC9480.16321274298985775No Hit
GTTCTGGATAGTAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA9110.15684262538371352No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACTACCGTG9100.15667046004300691No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9000.1549488066359409No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG8870.15271065720675508No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8740.15047250777756926No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGTTGGATCTTC8610.14823435834838344No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT8450.14547971289707784No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAC8400.14461888619354482No Hit
CCTAATAACTCTGTCTACTTGGGTGGGTGGTAATTTGGAAGATCCAACAT7680.13222298166266955No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA7460.12843534416712435No Hit
ATTCTATGCTGGGGGGACCTAATAACTCTGTCTACTTGGGTGGGTGGTAA7420.12774668280429793No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT7390.12723018678217812No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC7360.12671369076005834No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7320.12602502939723192No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC7200.12395904530875272No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7180.1236147146273395No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT7080.12189306122027349No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6990.1203435731539141No Hit
ATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGG6840.11776109304331508No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA6680.11500644759200947No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATCCGCACTCCTCCAGC6660.11466211691059626No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC6640.11431778622918307No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6390.11001365271151803No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6370.10966932203010483No Hit
TCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAGA6340.10915282600798502No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6290.10829199930445202No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACTTGATGGGCGTTC6210.10691467657879923No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT6170.10622601521597282No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAGTAAGGTTTAATAC6120.10536518851243981No Hit
GGCCAAGACTGTGCAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATT6090.10484869249032No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6070.1045043618089068No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG5970.1027827084018408No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5920.10192188169830779No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5900.1015775510168946No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5820.10020022829124178No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5810.10002806295053518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)