FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506822
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24500.4834044299576577No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21240.41908204458369996No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14920.2943834324476838No Hit
CCTAGTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC14580.2876749628074551No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC12690.2503837639250072No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12290.24249144670120873No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCAAAGAATCTCCTCC12170.24012375153406917No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11880.23440182154681527No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11860.2340072056856253No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11580.22848258362896637No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10640.20993563815303995No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTTACATAAGAGGACTCTTG10020.19770254645615223No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8860.17481482650713662No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8710.1718552075482122No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8590.16948751238107265No Hit
CTGTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8440.16652789342214822No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACACGAAATGTGAAACCACA7990.1576490365453749No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA7970.157254420684185No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT7940.15666249689240008No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTGGGATAAAACCTAGCAGG7940.15666249689240008No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7920.15626788103121017No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7900.15587326517002023No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA7760.15311095414169076No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7520.14837556380741168No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA7500.14798094794622174No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7470.14738902415443686No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7360.14521863691789227No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7350.1450213289872973No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC6990.13791824348587867No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA6940.13693170383290387No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6730.13278823729040964No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTGGGATAAAAC6700.13219631349862476No Hit
CAAAGAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTT6620.1306178500538651No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6460.12746092316434568No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6420.12667169144196583No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6380.12588245971958598No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6330.12489592006661115No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6240.12312014869125648No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6140.12114706938530688No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6010.11858206628757237No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5930.11700360284281265No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5840.115227831467458No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5800.11443859974507817No Hit
GTCCAAGAGTCCTCTTATGTAACAGAGAGTCCAAGAGTCCTCTTATGTAA5750.11345206009210335No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA5740.11325475216150838No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5710.1126628283697235No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5680.1120709045779386No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG5680.1120709045779386No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5680.1120709045779386No Hit
CTTATAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCC5670.11187359664734364No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5650.11147898078615372No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5650.11147898078615372No Hit
TGGGCATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTA5650.11147898078615372No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5620.11088705699436882No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5620.11088705699436882No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5490.10832205389663434No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC5460.10773013010484944No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5450.10753282217425446No Hit
CTCCTAGTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAG5440.1073355142436595No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG5390.10634897459068471No Hit
TGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTT5340.10536243493770989No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5280.10417858735414011No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5260.1037839714929502No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5230.1031920477011653No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5220.10299473977057035No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5170.10200820011759552No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT5140.10141627632581063No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5120.10102166046462072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)