FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506822
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24360.48064211892932823No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21030.4149385780412058No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14590.28787227073805005No Hit
CCTAGTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC13120.2588680049405906No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12860.25373799874512154No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11660.23006104707372607No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11280.22256334571111752No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCAAAGAATCTCCTCC10970.21644679986267368No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10800.21309256504255933No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTTACATAAGAGGACTCTTG10180.20085947334567167No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACACGAAATGTGAAACCACA9930.1959267750807976No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9850.19434831163603788No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9520.18783714992640413No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9440.18625868648164445No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA8620.17007943617285753No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA8400.16573866169976836No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8350.16475212204679354No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8270.16317365860203387No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC8260.1629763506714389No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTGGGATAAAACCTAGCAGG8070.1592274999901346No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT7880.15547864930883032No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7770.15330826207228573No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC7620.1503486431133613No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7610.15015133518276635No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTGGGATAAAAC7420.14640248450146207No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7190.14186440209777793No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA7180.14166709416718296No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7070.13949670693063837No Hit
CTGTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7060.1392993990000434No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA6850.1351559324575492No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6800.13416939280457438No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6780.13377477694338447No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6730.13278823729040964No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6720.13259092935981467No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6690.13199900556802982No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA6650.13120977384564994No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6630.13081515798446003No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6460.12746092316434568No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6420.12667169144196583No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6290.1241066883442313No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6130.12094976145471191No Hit
CTCCTAGTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAG6020.11877937421816732No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5980.11799014249578747No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5870.1158197552592429No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5790.11424129181448318No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5750.11345206009210335No Hit
CTTATAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCC5750.11345206009210335No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5740.11325475216150838No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG5730.11305744423091343No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5680.1120709045779386No Hit
TGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTT5640.11128167285555876No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5580.11009782527198898No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5540.10930859354960913No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC5520.1089139776884192No Hit
TGGGCATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTA5460.10773013010484944No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5460.10773013010484944No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5460.10773013010484944No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5400.10654628252127966No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5360.10575705079889981No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT5330.10516512700711493No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC5320.10496781907651997No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5220.10299473977057035No Hit
CAAAGAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTT5200.10260012390938042No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5200.10260012390938042No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG5160.10181089218700057No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT5100.10062704460343079No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT5070.1000351208116459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)