FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489752
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA22830.46615429850209905No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18350.37467942958885314No Hit
ATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11620.2372629412437315No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9670.19744687106943923No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT9610.19622176121792254No Hit
CACTAATATAGCCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9570.1954050213169114No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9530.1945882814159003No Hit
CTCTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9240.18866691713356964No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9240.18866691713356964No Hit
CTCTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCC9160.1870334373315474No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8340.17029026936081934No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC8190.16722749473202764No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8180.16702330975677485No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8050.1643689050784887No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT7850.1602852055734331No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC7830.1598768356229275No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7520.1535471013900913No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC7370.1504843267612996No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7200.14701318218200232No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC7090.14476714745422173No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6930.14150018785017723No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6830.13945833809764943No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC6770.13823322824613274No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT6480.13231186396380207No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6260.1278197945082409No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6100.1245528349041964No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT5930.12108169032489914No Hit
CTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5900.1204691353991408No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT5850.11944821052287688No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5830.11903984057237131No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5820.11883565559711853No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGTAA5770.11781473072085465No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5670.11577288096832683No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5550.11332266126529345No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5400.11025988663650174No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT5240.10699292703245725No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCCGC5160.10535944723043499No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5080.10372596742841275No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5050.10311341250265439No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA4970.10147993270063216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)