FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489752
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21540.4398144366944903No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18280.3732501347620837No Hit
ATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11330.2313415769614009No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10980.22419510282755353No Hit
CTCTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9750.19908035087146148No Hit
CACTAATATAGCCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9690.19785524101994478No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT9570.1954050213169114No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9520.1943840964406475No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9020.18417484767800846No Hit
CTCTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCC8590.17539489374213887No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8460.17274048906385273No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8240.16824841960829154No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8060.1645730900537415No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC7980.16293961025171924No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC7540.15395547134059687No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT7420.1515052516375635No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC7400.15109688168705793No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7320.1494634018850357No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7270.14844247700877178No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7000.1429294826769467No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6900.1408876329244189No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6900.1408876329244189No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6880.14047926297391333No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT6780.13843741322138553No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC6670.13619137849360494No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5970.12189843022591025No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC5780.11801891569610742No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5760.11761054574560185No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT5750.11740636077034908No Hit
CTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5720.11679380584459073No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5590.11413940116630457No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT5430.11087244156226007No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5430.11087244156226007No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCCGC5250.10719711200771002No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGTAA5160.10535944723043499No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT5160.10535944723043499No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5140.10495107727992944No Hit
CTCCTATGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGC5140.10495107727992944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)