FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468702
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA39590.8446731611983733No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21690.46276738738046774No Hit
CACTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACATTTCTT14120.30125751543624735No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13030.27800180071772684No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC12530.26733404167253394No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12440.26541384504439924No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11590.24727865466757126No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT11030.23533076453695523No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10990.23447734381333982No Hit
CATTTACACCAAGACATTATCAAAAAGTGCGAACAATTTGTAGGCCCACT10960.23383727827062825No Hit
CTCTATGACTATTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10950.2336239230897244No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10930.23319721272791666No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC10790.23021024019526265No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10710.2285033987480318No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10440.22274280886362763No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10350.22082261223549293No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT10120.21591544307470417No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10110.2157020878938003No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9770.20844801174306915No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT9350.19948709414510712No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9340.19927373896420328No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9340.19927373896420328No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9220.196713476793357No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9170.1956467008888377No Hit
ATATACATTTACACCAAGACATTATCAAAAAGTGCGAACAATTTGTAGGC8920.19031282136624125No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8920.19031282136624125No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA8680.18519229702454865No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8660.18476558666274093No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8550.1824186796727985No Hit
CAATAGGCCTGTTAACAGGTAGTTTTCTAAAACATTCTTTGATTTTCTGT8320.17751151051200975No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8270.17644473460749047No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCCAATATACA8030.1713242102657979No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7970.17004407918037476No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC7930.16919065845675932No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7890.16833723773314388No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7860.1676971721904323No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7830.16705710664772072No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7830.16705710664772072No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT7780.16599033074320144No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7700.16428348929597056No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7640.16300335821054743No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7550.1610831615824127No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT7480.1595896753160857No Hit
GTTAACAGGTAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT7470.15937632013518185No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7240.1544691509743931No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC7100.1514821784417391No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7000.14934862663270052No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6840.1459349437382388No Hit
GTTATGTCAACACTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTT6730.14358803674829637No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA6680.1425212608437771No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6670.1423079056628732No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6580.1403877090347385No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6580.1403877090347385No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC6320.1348404743312382No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6260.13356034324581503No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6210.13249356734129575No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAACTCTGCAAG6200.13228021216039187No Hit
GTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCCAATATACATTTACA6170.1316401466176803No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6060.1292932396277379No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT6040.12886652926593015No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5990.12779975336141086No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5960.1271596878186993No Hit
GTATCGGGAAGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG5950.12694633263779542No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5950.12694633263779542No Hit
CTCACAGTCAATGAAAAAAGGAGACTGCAATTGATTATGCCTGCTAGGTT5920.12630626709508386No Hit
TTCTTCCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTC5880.12545284637146845No Hit
ACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAATTCAGGCAA5860.12502613600966073No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5830.12438607046694915No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5820.1241727152860453No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5810.12395936010514144No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5760.12289258420062216No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5760.12289258420062216No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAATCTCCTGA5680.12118574275339128No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5680.12118574275339128No Hit
GTGTAAATGTATATTGGGAAAAAAGGGTGTTTTCCAATGAGGGTTAAAGA5620.11990561166796813No Hit
CTCCGAAAGACACCAAATACTCTATGACTATTTCTCTTCCAAAAGTGAGA5610.11969225648706426No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5590.11926554612525656No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA5540.11819877022073727No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5530.11798541503983341No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5510.1175587046780257No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5470.11670528395441027No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5430.11585186323079483No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAG5430.11585186323079483No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5360.11435837696446784No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5310.11329160105994854No Hit
ATCAAAAAGTGCGAACAATTTGTAGGCCCACTCACAGTCAATGAAAAAAG5290.11286489069814083No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5260.11222482515542925No Hit
CACCAAGACATTATCAAAAAGTGCGAACAATTTGTAGGCCCACTCACAGT5260.11222482515542925No Hit
CCAAATACTCTATGACTATTTCTCTTCCAAAAGTGAGACAAGAAATGTGA5240.11179811479362152No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5210.11115804925090995No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5210.11115804925090995No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT5180.11051798370819838No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5170.11030462852729453No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5160.11009127334639067No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5140.10966456298458296No Hit
GCTTTATTCTTCCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5080.10838443189915982No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5080.10838443189915982No Hit
ATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGGAACTAATGACTCTA5030.1073176559946405No Hit
CACCAAATACTCTATGACTATTTCTCTTCCAAAAGTGAGACAAGAAATGT5030.1073176559946405No Hit
TCACCATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGGAACTAATGA5030.1073176559946405No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5030.1073176559946405No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5020.10710430081373667No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5020.10710430081373667No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5010.1068909456328328No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4980.10625088009012124No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGTAGTTTTCTAAAAC4960.10582416972831352No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4920.10497074900469808No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4920.10497074900469808No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4910.10475739382379422No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4900.10454403864289037No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC4890.10433068346198651No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTGTACCTGTC4890.10433068346198651No Hit
TTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4870.10390397310017878No Hit
GTATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGA4870.10390397310017878No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTG4860.10369061791927493No Hit
CCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCCAAT4860.10369061791927493No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC4810.10262384201475563No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4790.10219713165294793No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4780.10198377647204407No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA4780.10198377647204407No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG4750.10134371092933249No Hit
CTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAATAGTCAT4710.10049029020571708No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTCCTTTTTTCA4690.10006357984390934No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC4690.10006357984390934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)