FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468702
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA35320.7535704989524261No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC22300.47578205341560315No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14920.31832592990855596No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13880.29613699109455477No Hit
CATTTACACCAAGACATTATCAAAAAGTGCGAACAATTTGTAGGCCCACT12160.25943989997909117No Hit
CTCTATGACTATTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11980.2555995067228217No Hit
CACTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACATTTCTT11940.25474608599920634No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11230.2395978681550324No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11110.2370376059841861No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10650.22722326766260867No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT10550.22508971585357007No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10510.22423629512995463No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10220.21804899488374277No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10200.21762228452193505No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC10070.2148486671701849No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9910.2114349842757232No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9530.20332748740137657No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT9500.20268742185866498No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9280.19799360787878012No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9230.19692683197426084No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9110.19436656980341452No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC9010.19223301799437598No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT9010.19223301799437598No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT9000.1920196628134721No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8900.1898861110044335No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8820.18817926955720266No Hit
ATATACATTTACACCAAGACATTATCAAAAAGTGCGAACAATTTGTAGGC8630.18412552112002936No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8570.1828453900346062No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8320.17751151051200975No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8240.1758046690647789No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8030.1713242102657979No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA8000.17068414472308632No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCCAATATACA7790.16620368592410528No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC7660.16343006857235515No Hit
CAATAGGCCTGTTAACAGGTAGTTTTCTAAAACATTCTTTGATTTTCTGT7610.16236329266783583No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG7560.16129651676331658No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7500.16001638567789342No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7470.15937632013518185No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7390.15766947868795098No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT7250.15468250615529697No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7200.15361573025077768No Hit
CTCCGAAAGACACCAAATACTCTATGACTATTTCTCTTCCAAAAGTGAGA7010.1495619818136044No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT6920.14764178518546967No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6810.14529487819552722No Hit
GTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCCAATATACATTTACA6770.1444414574719118No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6770.1444414574719118No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC6750.14401474711010406No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6670.1423079056628732No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6620.14124112975835393No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6560.13996099867293077No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6510.13889422276841148No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6470.13804080204479605No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT6450.13761409168298833No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6430.13718738132118063No Hit
GTTAACAGGTAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT6410.1367606709593729No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6310.13462711915033435No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6240.1331336328840073No Hit
TTCTTCCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTC6130.13078672589406487No Hit
CTCACAGTCAATGAAAAAAGGAGACTGCAATTGATTATGCCTGCTAGGTT6120.13057337071316102No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6110.13036001553225718No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC6010.12822646372321858No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5970.12737304299960317No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5950.12694633263779542No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAACTCTGCAAG5950.12694633263779542No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5900.12587955673327617No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5830.12438607046694915No Hit
CAAATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC5800.12374600492423757No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5710.12182580829610284No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5670.12097238757248742No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAATCTCCTGA5660.12075903239158357No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5660.12075903239158357No Hit
GTTATGTCAACACTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTT5620.11990561166796813No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5550.11841212540164112No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTG5470.11670528395441027No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5470.11670528395441027No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5460.1164919287735064No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5450.11627857359260256No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5440.11606521841169869No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5410.11542515286898712No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5410.11542515286898712No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5410.11542515286898712No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA5400.11521179768808326No Hit
CACCAAGACATTATCAAAAAGTGCGAACAATTTGTAGGCCCACTCACAGT5290.11286489069814083No Hit
ACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAATTCAGGCAA5230.11158475961271769No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5220.11137140443181381No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5210.11115804925090995No Hit
GTATCGGGAAGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG5200.11094469407000611No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5200.11094469407000611No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5190.11073133888910225No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5150.10987791816548681No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5140.10966456298458296No Hit
GTGTAAATGTATATTGGGAAAAAAGGGTGTTTTCCAATGAGGGTTAAAGA5140.10966456298458296No Hit
CCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCCAAT5140.10966456298458296No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5100.10881114226096752No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC5090.10859778708006365No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5060.10795772153735209No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA5060.10795772153735209No Hit
GCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG5030.1073176559946405No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5000.10667759045192894No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4990.1064642352710251No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGTAGTTTTCTAAAAC4980.10625088009012124No Hit
TCACCATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGGAACTAATGA4970.10603752490921736No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTGTACCTGTC4970.10603752490921736No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCATCAATTCTTGCCC4880.10411732828108265No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4860.10369061791927493No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4850.10347726273837107No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4830.10305055237656337No Hit
ATCAAAAAGTGCGAACAATTTGTAGGCCCACTCACAGTCAATGAAAAAAG4810.10262384201475563No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC4810.10262384201475563No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA4790.10219713165294793No Hit
ATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGGAACTAATGACTCTA4750.10134371092933249No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTCCTTTTTTCA4750.10134371092933249No Hit
CCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTT4740.10113035574842864No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4730.10091700056752478No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4690.10006357984390934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)