FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370427
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21830.5893198929883621No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACGTTGACAGACT11600.31315211904099866No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10750.29020562755954615No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT10640.28723608160312286No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10020.27049864075782815No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9890.26698917735478245No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9800.26455954884498156No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9030.24377272715001874No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAC8800.23756367651386104No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACT8550.23081470843108087No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT8150.22001635949863266No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7600.20516862971651637No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7600.20516862971651637No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7540.20354887737664912No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7480.20192912503678187No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6930.18708139525466555No Hit
ATGTAAGACCTTGGGCAAGAATGGATGGGCGTTCACGGTGGTCTCCATGC6850.18492172546817592No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6660.179792509725263No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6630.1789826335553294No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6460.1743933352590389No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6260.1689941607928148No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6150.1660246148363915No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATGGATGGGCGTTC6140.1657546561130803No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6040.16305506887996826No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT5980.16143531654010101No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5920.15981556420023377No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5800.15657605952049933No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAAT5710.15414643101069847No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5600.1511768850542752No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5560.15009705016103037No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5480.1479373803745407No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5470.14766742165122954No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5320.14361804080156143No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTACCAC5280.14253820590831662No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5220.1409184535684494No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTGAAACATTCTTTGATTTTCTGT5190.14010857739851576No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5150.13902874250527095No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5140.13875878378195974No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5120.1382188663353373No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5090.1374089901654037No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5060.1365991139954701No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACG5010.13524932037891407No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4940.13335960931573562No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4900.13227977442249078No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC4750.1282303935728227No Hit
GGCTATGTCATTGGATGTTATGGGTCATTACCACAAGATCACATCATACA4630.12499088889308825No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4620.12472093016977705No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4600.12418101272315464No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4560.12310117782990981No Hit
GTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGA4550.1228312191065986No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4550.1228312191065986No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4550.1228312191065986No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4540.1225612603832874No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGACTCCACCCCAAAAGGCCTCCGT4540.1225612603832874No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4480.12094150804342016No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4460.12040159059679774No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4460.12040159059679774No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG4450.12013163187348654No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4420.11932175570355294No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4410.11905179698024172No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4340.11716208591706329No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT4310.11635220974712966No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4310.11635220974712966No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4300.11608225102381846No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4300.11608225102381846No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCCGGTCTGTGCCAAGTGTTTGCTG4280.11554233357719604No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTACCA4270.11527237485388483No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC4260.11500241613057363No Hit
TCTGCGTACACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCCG4220.11392258123732882No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT4210.11365262251401761No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4210.11365262251401761No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA4180.11284274634408399No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC4170.11257278762077279No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT4170.11257278762077279No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4160.11230282889746158No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4100.11068307655759434No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4100.11068307655759434No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT4090.11041311783428313No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4070.10987320038766071No Hit
ATGTCATTGGATGTTATGGGTCATTACCACAAGATCACATCATACAGAAA4070.10987320038766071No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4060.10960324166434951No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC4010.10825344804779351No Hit
GTTATGGGTCATTACCACAAGATCACATCATACAGAAAATCAAAGAATGT3960.10690365443123746No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT3920.10582381953799264No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC3920.10582381953799264No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG3920.10582381953799264No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA3890.10501394336805903No Hit
GTATGATGTGATCTTGTGGTAATGACCCATAACATCCAATGACATAGCCC3870.1044740259214366No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA3830.10339419102819178No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3830.10339419102819178No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA3820.10312423230488059No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3800.10258431485825818No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTCTTTTGGGTTTT3790.10231435613494698No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3750.10123452124170215No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3750.10123452124170215No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3720.10042464507176853No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA3710.10015468634845733No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT3710.10015468634845733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)