FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426692
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA26230.6147291254581759No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT17940.42044378615019734No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGGTACTCTTTACATTTTAT16700.3913830116336842No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14300.33513635127914276No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC13860.32482446354747685No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13420.31451257581581094No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT12280.28779541214740373No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12020.2817020239423284No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11830.27724916333092725No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT11820.27701480224611663No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT11600.2718588583802837No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10850.25428177701948945No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10350.24256372277896002No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9900.23201747396248348No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9370.21959633646752222No Hit
CTCTATGACCGTTTCTCTTCCAAAGGTGAGACAAGAAATGTGAAACCACA8770.20553467137888687No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8740.2048315881244551No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT8740.2048315881244551No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8630.20225361619153862No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCTCTGGATATTGGATCTTC8580.20108181076748566No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGTTCATTGCCACAA8510.19944128317381155No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8480.1987381999193798No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8270.1938166171383574No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8220.19264481171430448No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7490.17553645252313144No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC7470.17506773035351025No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7460.17483336926869966No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA7180.16827125889400318No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7180.16827125889400318No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTACGTTGGCGA7140.16733381455476082No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG7020.16452148153703372No Hit
ATGTCATTGGATGTTATGGTTCATTGCCACAAGATCACATCATACAGAAA6950.1628809539433596No Hit
CTCTAAGGCTTCCCGATACAAAGCTGAGGCGGTATCTAGAAGATCTCGTA6900.16170914851930668No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCGGGAT6780.15889681550157958No Hit
ATGTAAAGAGTACCCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATAC6770.15866245441676902No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGCATTAAACCTTAT6760.15842809333195843No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6700.15702192682309488No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGTTCATTGCCAC6680.1565532046534737No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT6680.1565532046534737No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA6580.1542095938053678No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA6430.15069417753320896No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6390.1497567331939666No Hit
GGCTTTCACTTTCTCGCCAACGTACAAGGCCTTTCTGTGTAAACAATACC6360.14905364993953485No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6360.14905364993953485No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6360.14905364993953485No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6220.1457725947521866No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6120.14342898390408068No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATATTTAATCT6070.14225717848002775No Hit
CTTCTAGATACCGCCTCAGCTTTGTATCGGGAAGCCTTAGAGTCTCCTGA5960.13967920654711127No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5920.1387417622078689No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA5740.1345232626812783No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5700.13358581834203595No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5610.13147656857874063No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACTGCACTCAGGCA5580.13077348532430888No Hit
ACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG5540.12983604098506651No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5530.12960167990025592No Hit
GTATGATGTGATCTTGTGGCAATGAACCATAACATCCAATGACATAGCCC5520.12936731881544533No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT5520.12936731881544533No Hit
AGATAAGGGCATTAAACCTTATTATCCCGAACATCTAGTTAATCATTACT5510.12913295773063474No Hit
CCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC5490.12866423556101356No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5490.12866423556101356No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5450.12772679122177122No Hit
GAACCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTACCCCCA5390.12632062471290767No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5390.12632062471290767No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5340.1251488192888547No Hit
TTATGGTTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT5310.12444573603442295No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5280.12374265277999119No Hit
TGTTTACACAGAAAGGCCTTGTACGTTGGCGAGAAAGTGAAAGCCTGCTT5240.12280520844074883No Hit
GATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGT5230.12257084735593825No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5220.12233648627112766No Hit
ATGTTATGGTTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAAT5220.12233648627112766No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5210.12210212518631706No Hit
ATGTGATCTTGTGGCAATGAACCATAACATCCAATGACATAGCCCATAAA5190.1216334030166959No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC5140.12046159759264294No Hit
GGATAATAAGGTTTAATGCCCTTATCTAATGGCAAATATTTGGTAACATT5130.12022723650783236No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5130.12022723650783236No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5090.11928979216859No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5090.11928979216859No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5070.1188210699989688No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT5070.1188210699989688No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5040.11811798674453704No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG5030.11788362565972645No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG5010.11741490349010528No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5010.11741490349010528No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4980.1167118202356735No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA4940.11577437589643115No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC4920.11530565372680998No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4870.11413384830275702No Hit
ATCCAATATCCAGAGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC4870.11413384830275702No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4840.11343076504832525No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGGTACT4830.11319640396351466No Hit
TAACATAACTGACTACTAGGTCTCTGGATATTGGATCTTCCAAATTACCA4810.11272768179389349No Hit
GGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGGTACTCTTTACA4790.11225895962427232No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC4780.11202459853946173No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC4780.11202459853946173No Hit
GGGGTACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGTTCAT4760.11155587636984055No Hit
CTCACAGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT4750.11132151528502995No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4710.11038407094578759No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAGCA4680.10968098769135583No Hit
CACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4610.1080404600976817No Hit
GGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGGTACTCTTTAC4610.1080404600976817No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA4610.1080404600976817No Hit
ACATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCG4580.10733737684324994No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTA4550.10663429358881817No Hit
ACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAA4540.10639993250400757No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4500.10546248816476521No Hit
CTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA4500.10546248816476521No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG4500.10546248816476521No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTTTA4490.10522812707995463No Hit
AATGTAAAGAGTACCCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATA4470.10475940491033345No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4440.10405632165590167No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4440.10405632165590167No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT4430.10382196057109108No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGT4420.1035875994862805No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG4420.1035875994862805No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGGCAGACGGAGAAGG4400.10311887731665932No Hit
GTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC4390.10288451623184874No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC4360.10218143297741696No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAGGTGAGA4340.10171271080779579No Hit
CTACTAGGTCTCTGGATATTGGATCTTCCAAATTACCACCCACCCAGGTG4340.10171271080779579No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT4330.1014783497229852No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4310.10100962755336403No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4310.10100962755336403No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4310.10100962755336403No Hit
GGCTATGTCATTGGATGTTATGGTTCATTGCCACAAGATCACATCATACA4290.10054090538374283No Hit
GTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4270.10007218321412166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)