FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485262
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46200.9520630092609765No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT27020.5568126084465711No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT24290.5005543397175134No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23560.48551091987421224No Hit
CATTTAAACCCTAACAAAACACAAAGATGGGGTTACTCTTTACATTTCAT22870.4712917970086263No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG20320.4187428646792867No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT20120.414621379790711No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT19800.40802700396898994No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA19240.3964868462809781No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTAT15880.3272459001529071No Hit
GGATATGTCATTGGATGTTATGGATCATTGCCGCAAGATCACATCATACA14880.3066384757100288No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGCAGCA13930.28706142248929445No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT13020.2683086662462752No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG12490.2573867312915497No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC11980.2468769448256818No Hit
CTCTTTACATTTCATGGGATATGTCATTGGATGTTATGGATCATTGCCGC11970.246670870581253No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG11690.2409007917372471No Hit
GACATATCCCATGAAATGTAAAGAGTAACCCCATCTTTGTGTTTTGTTAG11680.24069471749281832No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA11620.23945827202624562No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11420.23533678713766995No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11230.2314213764935231No Hit
GGGTTACTCTTTACATTTCATGGGATATGTCATTGGATGTTATGGATCAT10980.2262695203828035No Hit
GAATACATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA10920.2250330749162308No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT10880.22420877793851568No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCGTCAACCACC10820.222972332471943No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG10690.2202933672943688No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT10690.2202933672943688No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG10460.21555365967250684No Hit
GTTATGGATCATTGCCGCAAGATCACATCATACAGAAAATCAAAGAATGT10070.2075167641397843No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG10060.2073106898953555No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10030.20669246716206918No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC9990.205868170184354No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAA9900.20401350198449497No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG9890.2038074277400662No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG9880.2036013534956374No Hit
GCTTAGATTGAATACATGCATATAAGGGCATTAAAGCAGGATAACCACAT9820.2023649080290647No Hit
GATCCATAACATCCAATGACATATCCCATGAAATGTAAAGAGTAACCCCA9800.20195275954020714No Hit
GAGTAACCCCATCTTTGTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA9760.20112846256249203No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC9710.20009809134034812No Hit
ACTCTTTACATTTCATGGGATATGTCATTGGATGTTATGGATCATTGCCG9670.19927379436263296No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT9530.19638875494063002No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9480.1953583837184861No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC9220.19000045336333773No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9120.18793971091904993No Hit
GTCCCGTGCTGGTGGTTGACGATCCTGGAATTAGAGGACAAACGGGCAAC9030.18608504271919085No Hit
GGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8980.18505467149704696No Hit
ACACAAAGATGGGGTTACTCTTTACATTTCATGGGATATGTCATTGGATG8890.1832000032971879No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA8830.1819635578306152No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG8760.18052103811961373No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC8590.17701777596432444No Hit
GTATGATGTGATCTTGCGGCAATGATCCATAACATCCAATGACATATCCC8540.17598740474218053No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC8480.1747509592756078No Hit
ATCCATAACATCCAATGACATATCCCATGAAATGTAAAGAGTAACCCCAT8400.17310236532017756No Hit
TTATGGATCATTGCCGCAAGATCACATCATACAGAAAATCAAAGAATGTT8350.17207199409803364No Hit
ACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTT8110.16712621223174284No Hit
GATTGAATACATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGT8070.1663019152540277No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACACAAAGATGGGGTTACT7940.16362295007645355No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7930.16341687583202477No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7880.16238650460988086No Hit
GCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGCAGC7590.15641035152144614No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7570.15599820303258857No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG7420.15290708936615682No Hit
CATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG7360.15167064389958415No Hit
GCTTTAATGCCCTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7200.14837345598872362No Hit
GTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7200.14837345598872362No Hit
CATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGC7040.14507626807786309No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA6930.14280945138914647No Hit
GTAAAGAGTAACCCCATCTTTGTGTTTTGTTAGGGTTTAAATGTATACCC6850.14116085743371623No Hit
ATGTAAAGAGTAACCCCATCTTTGTGTTTTGTTAGGGTTTAAATGTATAC6790.13992441196714353No Hit
ATGTCATTGGATGTTATGGATCATTGCCGCAAGATCACATCATACAGAAA6750.13910011498942837No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATTCAATCTAAGC6720.13848189225614205No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC6690.1378636695228557No Hit
CACTTGGCACAGACCGGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGT6690.1378636695228557No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT6670.13745152103399813No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6510.1341543331231376No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT6500.13394825887870881No Hit
CTTTACATTTCATGGGATATGTCATTGGATGTTATGGATCATTGCCGCAA6480.13353611038985125No Hit
AAGATGGGGTTACTCTTTACATTTCATGGGATATGTCATTGGATGTTATG6470.13333003614542246No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT6420.13229966492327855No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA6410.13209359067884976No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT6410.13209359067884976No Hit
GGGTATACATTTAAACCCTAACAAAACACAAAGATGGGGTTACTCTTTAC6400.13188751643442098No Hit
GTCATTGGATGTTATGGATCATTGCCGCAAGATCACATCATACAGAAAAT6380.13147536794556341No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT6370.13126929370113463No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA6290.1296206997457044No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6240.12859032852356045No Hit
AACACAAAGATGGGGTTACTCTTTACATTTCATGGGATATGTCATTGGAT6210.12797210579027413No Hit
ATGTGATCTTGCGGCAATGATCCATAACATCCAATGACATATCCCATGAA6180.12735388305698778No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT6050.1246749178794136No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6030.12426276939055603No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6000.12364454665726968No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT5990.1234384724128409No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5970.12302632392398333No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG5930.12220202694626821No Hit
ATAACATCCAATGACATATCCCATGAAATGTAAAGAGTAACCCCATCTTT5880.12117165572412428No Hit
CTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTT5880.12117165572412428No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATATAAGGGCATTAAAGCAGG5810.11972913601312281No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5740.11828661630212134No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5740.11828661630212134No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5720.11787446781326377No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5710.11766839356883498No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5630.11601979961340472No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT5630.11601979961340472No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCGGGCC5570.11478335414683202No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5570.11478335414683202No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5510.11354690868025932No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC5510.11354690868025932No Hit
GGTATACATTTAAACCCTAACAAAACACAAAGATGGGGTTACTCTTTACA5500.11334083443583054No Hit
CCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCG5500.11334083443583054No Hit
CCCTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAAC5490.11313476019140176No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA5460.1125165374581154No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5450.11231046321368662No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC5410.1114861662359715No Hit
GGATGTTATGGATCATTGCCGCAAGATCACATCATACAGAAAATCAAAGA5400.11128009199154272No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5370.11066186925825637No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5320.10963149803611244No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG5320.10963149803611244No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT5280.10880720105839732No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5230.10777682983625342No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC5200.10715860710296707No Hit
CTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATT5170.1065403843696807No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA5140.10592216163639435No Hit
CCTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACT5090.10489179041425045No Hit
GTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTT5070.10447964192539289No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACA5050.10406749343653532No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5040.10386141919210654No Hit
GTCTGTCAACGTATTGTGGGTCTTTTGGGTTTTGCTGCTCCTTTTACACA5040.10386141919210654No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5010.10324319645882017No Hit
GCATGGTCCCGTGCTGGTGGTTGACGATCCTGGAATTAGAGGACAAACGG5010.10324319645882017No Hit
GTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATTCAATCTAAGCAG4980.10262497372553384No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT4960.10221282523667627No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4950.10200675099224749No Hit
CATTGGATGTTATGGATCATTGCCGCAAGATCACATCATACAGAAAATCA4950.10200675099224749No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT4940.1018006767478187No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC4930.10159460250338992No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCGTCAACCA4910.10118245401453235No Hit
ATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4900.10097637977010357No Hit
GACCGGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGTATTGTTTACAC4860.10015208279238845No Hit
CCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG4860.10015208279238845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)