FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485262
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45970.9473233016391145No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT30870.6361511925516525No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT25060.5164220565385297No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG23790.4902506274960743No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23460.4834501774299244No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT23160.47726795009706097No Hit
CATTTAAACCCTAACAAAACACAAAGATGGGGTTACTCTTTACATTTCAT20950.43172554207829994No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA20360.4195671616570018No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT18850.3884499507482556No Hit
GGATATGTCATTGGATGTTATGGATCATTGCCGCAAGATCACATCATACA15140.31199640606517715No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTAT14790.30478380751016976No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA13830.2850006800450066No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC12560.2588292510025512No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGCAGCA12130.2499680584921135No Hit
CTCTTTACATTTCATGGGATATGTCATTGGATGTTATGGATCATTGCCGC12070.24873161302554084No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG12000.24728909331453935No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT11350.23389426742666847No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC11350.23389426742666847No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG11330.2334821189378109No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT11300.23286389620452455No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT11110.22894848556037772No Hit
GGGTTACTCTTTACATTTCATGGGATATGTCATTGGATGTTATGGATCAT10820.222972332471943No Hit
GCTTAGATTGAATACATGCATATAAGGGCATTAAAGCAGGATAACCACAT10640.21926299607222488No Hit
GTTATGGATCATTGCCGCAAGATCACATCATACAGAAAATCAAAGAATGT10510.21658403089465073No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG10420.2147293626947917No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAA10340.2130807687393614No Hit
GACATATCCCATGAAATGTAAAGAGTAACCCCATCTTTGTGTTTTGTTAG10330.21287469449493263No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10210.21040180356178723No Hit
GAATACATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA10160.20937143233964334No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC10080.20772283838421307No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC10080.20772283838421307No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10080.20772283838421307No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG10020.20648639291764034No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG9870.20339527925120862No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG9850.20298313076235106No Hit
GTATGATGTGATCTTGCGGCAATGATCCATAACATCCAATGACATATCCC9840.20277705651792227No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCGTCAACCACC9820.2023649080290647No Hit
GGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG9690.19968594285149055No Hit
GTCCCGTGCTGGTGGTTGACGATCCTGGAATTAGAGGACAAACGGGCAAC9630.19844949738491782No Hit
GAGTAACCCCATCTTTGTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA9380.19329764127419827No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC9190.18938223063005138No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG8970.18484859725261818No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACACAAAGATGGGGTTACT8960.1846425230081894No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG8690.17907851840861225No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC8670.17866636991975468No Hit
ACTCTTTACATTTCATGGGATATGTCATTGGATGTTATGGATCATTGCCG8580.17681170171989566No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8550.1761934789866093No Hit
TTATGGATCATTGCCGCAAGATCACATCATACAGAAAATCAAAGAATGTT8490.17495703352003658No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8470.17454488503117904No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8440.1739266622978927No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA8420.17351451380903513No Hit
GTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA8110.16712621223174284No Hit
GATCCATAACATCCAATGACATATCCCATGAAATGTAAAGAGTAACCCCA7940.16362295007645355No Hit
GATTGAATACATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGT7810.16094398489887937No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7610.1568225000103037No Hit
GCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGCAGC7580.15620427727701736No Hit
ATCCATAACATCCAATGACATATCCCATGAAATGTAAAGAGTAACCCCAT7550.155586054543731No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG7410.15270101512172807No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7310.15064027267744023No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA7300.15043419843301142No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC7240.14919775296643875No Hit
ACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTT7140.14713701052215092No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA7110.14651878778886457No Hit
ACACAAAGATGGGGTTACTCTTTACATTTCATGGGATATGTCATTGGATG7040.14507626807786309No Hit
CATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG7020.14466411958900552No Hit
GTAAAGAGTAACCCCATCTTTGTGTTTTGTTAGGGTTTAAATGTATACCC6920.14260337714471769No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6870.1415730059225738No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATTCAATCTAAGC6860.141366931678145No Hit
ATGTAAAGAGTAACCCCATCTTTGTGTTTTGTTAGGGTTTAAATGTATAC6780.13971833772271475No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC6760.13930618923385715No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6710.13827581801171326No Hit
CTTTACATTTCATGGGATATGTCATTGGATGTTATGGATCATTGCCGCAA6560.13518470434528151No Hit
CATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGC6530.13456648161199516No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT6510.1341543331231376No Hit
GCTTTAATGCCCTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTT6450.13291788765656493No Hit
GGGTATACATTTAAACCCTAACAAAACACAAAGATGGGGTTACTCTTTAC6390.1316814421899922No Hit
ATGTCATTGGATGTTATGGATCATTGCCGCAAGATCACATCATACAGAAA6320.13023892247899074No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT6280.1294146255012756No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6210.12797210579027413No Hit
CACTTGGCACAGACCGGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGT6190.12755995730141656No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT6150.12673566032370143No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT6090.12549921485712873No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6070.12508706636827116No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT6050.1246749178794136No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT6000.12364454665726968No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATATAAGGGCATTAAAGCAGG5990.1234384724128409No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5990.1234384724128409No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT5940.12240810119069699No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA5920.12199595270183942No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5910.12178987845741064No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5850.12055343299083793No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5800.11952306176869402No Hit
GTCATTGGATGTTATGGATCATTGCCGCAAGATCACATCATACAGAAAAT5790.11931698752426524No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT5780.11911091327983646No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5770.11890483903540768No Hit
AAGATGGGGTTACTCTTTACATTTCATGGGATATGTCATTGGATGTTATG5750.11849269054655012No Hit
ATGTGATCTTGCGGCAATGATCCATAACATCCAATGACATATCCCATGAA5730.11808054205769256No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5630.11601979961340472No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5560.11457727990240324No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC5490.11313476019140176No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC5470.11272261170254419No Hit
GGTATACATTTAAACCCTAACAAAACACAAAGATGGGGTTACTCTTTACA5440.11210438896925784No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCGGGCC5400.11128009199154272No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5390.11107401774711394No Hit
AACACAAAGATGGGGTTACTCTTTACATTTCATGGGATATGTCATTGGAT5360.11045579501382757No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG5330.10983757228054122No Hit
GTCTGTCAACGTATTGTGGGTCTTTTGGGTTTTGCTGCTCCTTTTACACA5300.10921934954725489No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5250.10818897832511097No Hit
GTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTT5220.10757075559182463No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT5210.10736468134739585No Hit
CTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTT5180.10674645861410949No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA5150.10612823588082314No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA5140.10592216163639435No Hit
GGATGTTATGGATCATTGCCGCAAGATCACATCATACAGAAAATCAAAGA5100.10509786465867924No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5100.10509786465867924No Hit
CCTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACT5050.10406749343653532No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5020.10344927070324898No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC5020.10344927070324898No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG5000.10303712221439139No Hit
GACCGGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGTATTGTTTACAC4950.10200675099224749No Hit
GTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATTCAATCTAAGCAG4930.10159460250338992No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCGTCAACCA4920.10138852825896114No Hit
ATAACATCCAATGACATATCCCATGAAATGTAAAGAGTAACCCCATCTTT4880.10056423128124602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)