FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372509
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19410.5210612361043625No Hit
CCCCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17920.4810622025239659No Hit
CTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACATAGCGCC16990.4560963627724431No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16910.45394876365403247No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14470.3884469905425104No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12190.32724041566780937No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11590.3111334222797301No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT10590.28428843329959813No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAAATCTGCAAG9510.2552958452010555No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9380.2518059966336384No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG8370.22469255776370506No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8200.22012890963708256No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCACGC8120.21798131051867206No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7490.20106896746118885No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCTCT7480.20080051757138756No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7320.19650531933456641No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT7110.19086787164873867No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6930.1860357736323149No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA6910.18549887385271227No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCTTCCACCAATCGTCAGTC6870.184425074293507No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6870.184425074293507No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6840.18361972462410306No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6710.18012987605668587No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCCCCAGC6420.1723448292524476No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6380.17127102969324232No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6370.17100257980344097No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC6330.16992878024423572No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6280.1685865307952291No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6220.1669758314564212No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6220.1669758314564212No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6140.1648282323380106No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6130.1645597824482093No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6070.16294908310940137No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG6060.16268063321960008No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6060.16268063321960008No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6000.16106993388079213No Hit
GGGTTTACTGTCCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5940.1594592345419842No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5880.1578485352031763No Hit
AGCTGGGGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5790.15543248619496441No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGGACAGTAAACCCTGTTCCGA5750.15435868663575913No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5690.15274798729695122No Hit
CTTTGGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5670.15221108751734858No Hit
GCCTGAGGATGCGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5590.15006348839893802No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5520.14818433917032878No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5510.14791588928052746No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5470.14684208972132218No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5460.14657363983152086No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5420.14549984027231558No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5410.14523139038251426No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5350.1436206910437063No Hit
CTCTGGGATCTTGCAGATTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG5340.143352241153905No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5330.1430837912641037No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5320.14281534137430238No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5190.1393254928068852No Hit
CTTCTACTGTTCCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCT5190.1393254928068852No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5160.13852014313748123No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5070.13610409412926935No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5000.13422494490066011No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5000.13422494490066011No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4860.13046664644344164No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4820.12939284688423636No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4740.1272452477658258No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4660.12509764864741524No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4650.1248291987576139No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4630.12429229897801128No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4580.12295004952900468No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4550.1221446998596007No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4540.12187624996979939No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTCGTTTA4530.12160780007999808No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4530.12160780007999808No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4500.1208024504105941No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4490.12053400052079279No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4470.11999710074119013No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGG4470.11999710074119013No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCTGTCCCGATAATGTTT4430.11892330118198488No Hit
CCATATTCTTGGGAACAAGAGATACAGCATGGGGCAGAATCTTTCCACCA4430.11892330118198488No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCTCTCACAGT4400.11811795151258091No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4380.11758105173297825No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC4340.11650725217377299No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4340.11650725217377299No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4320.11597035239417033No Hit
GTGGAAGCAGGAGGCGGATTTGTTGGCAAGGTTTGTAGTATGCCCTGAGC4320.11597035239417033No Hit
CAGTAAACCCTGTTCCGACTACTGTATCTCACATATCGTCAATCTTCTCG4300.11543345261456771No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4290.11516500272476639No Hit
GCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTAC4260.11435965305536243No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4230.11355430338595845No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4220.11328585349615714No Hit
CTTCTAGATACCGCCGCAGCTCTGTTTCGGGAAGCCTTAGAGTCTCCTGA4190.11248050382675318No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGAT4140.11113825437774658No Hit
GTGTTAACATAACTAACTACTAGGTCCCTGGATGTCGGATCTTCCAAATT4060.10899065525933603No Hit
GTCTCCACCTTTGAGAAACACGCATCCTCAGGCCATGCAGTGGAACTCCA4050.10872220536953468No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAA4050.10872220536953468No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGATTTTGGTGGAAGGT4040.10845375547973338No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG4040.10845375547973338No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC3990.10711150603072678No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC3940.10576925658172018No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA3930.10550080669191886No Hit
CTACTAGGTCCCTGGATGTCGGATCTTCCAAATTACCACCCACCCAGGTG3930.10550080669191886No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3930.10550080669191886No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3910.1049639069123162No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATGGACATTGA3910.1049639069123162No Hit
CTCTTCTACTGTTCCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC3900.10469545702251488No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3870.10389010735311092No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3850.10335320757350829No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3820.10254785790410433No Hit
CATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT3810.102279408014303No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG3800.10201095812450169No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCTTTAATCTAAGCTCCTCC3780.10147405834489906No Hit
ATCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA3750.10066870867549509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)