FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505064
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTTGTTTTGTCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAAT40360.7991066478703689No Hit
GGGACCTGGTAGTTAGTTATGTTAATACTAATATGGGCCTAAAGTTCAGG35820.7092170497204315No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG28290.5601270334056674No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA20300.40192926045016075No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC20080.39757337683937083No Hit
ACAAAAAGGTGGGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATG19820.39242551439025547No Hit
ACCTCCTTGTTTTGTCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAA19210.3803478371057925No Hit
ACCATATCCCTGGATGTTGGATCTTGCAAATTACCACCCACCCAGGTGGA19130.37876387942914164No Hit
ACCATATCCCTGGATGTTGCATCTTGCAAATTACCACCCACCCAGGTGGA18590.36807216511174823No Hit
ACAAAAAGGTGGGGTTACTCTTTACATTTCATGGGTTATGTCATTGGATG18210.36054836614765656No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCCACCGTG18050.35738045079435476No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18030.356984461375192No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC16420.32510731313259306No Hit
GTATCTTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCA15530.30748578397985205No Hit
CCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGACAAAAGAAAAT15500.306891799851108No Hit
GAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC14900.2950121172762264No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA14110.27937053521929894No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13530.26788684206358004No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACAAAAA13460.26650087909651055No Hit
GTTCAGCGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGTGAGAGA13330.26392694787195287No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12670.2508592970395831No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12430.24610742400963045No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG12370.2449194557521423No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC10930.21640821757242645No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC10710.21205233396163653No Hit
ATCTCTTGTTCCCAAGAATATGGTGACCCACAGAATGAGGCGCTATGTGTTGTTTCTCTCTTATATAAGATAC10530.20848842918917207No Hit
ACAAAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA10330.20452853499754486No Hit
GATTCATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCC10200.20195460377298718No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCAACAAACTCTGCAAG10150.20096463022508038No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT10130.2005686408059177No Hit
GGTATCTTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTC10110.20017265138675494No Hit
ATCTCTTGTTCCCAAGAATATGGTGACCCACAGAATGAGGCGCTATGTGT9900.1960147624855464No Hit
GTATCTTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGAGAT9880.19561877306638364No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG9860.19522278364722093No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCACAGAATGAGGCGCTATGT9780.19363882597057006No Hit
ATCTCTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGGAT9160.18136315397652575No Hit
GGATTCATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCC9030.17878922275196807No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9000.17819523862322398No Hit
CAATTACATAACCCATGAAATGTAAAGAGTAACCCCACCTTTTTGT8660.17146341849745775No Hit
TTCTCGTTGGTTCTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCA8650.17126542378787638No Hit
CAATTACATAACCCATGAAATGTAAAGAGTAACCCCACCTCCTTGTTTTG8340.16512758779085424No Hit
GGATAAGGGTATTAAACCTTATTATCCACAACATGTAGTTAATCATTACT8300.1643356089525288No Hit
TTTCCCTTCCAAAAGTGAGGCAAGAAATGTGAAACCACAATAGTTGCCTG8270.1637416248237847No Hit
GGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCC8260.16354363011420334No Hit
ACAAGGAGGTGGGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATG8230.16294964598545925No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC8210.16255365656629653No Hit
ACAAAAAGGTGGGGTTACTCTTTACATTTCATGGGTTATGTAATTG7900.1564158205692744No Hit
GGGATATGGTAGTTAGTTATGTTAATACTAATATGGGCCTAAAGTTCAGG7840.1552278523117862No Hit
ACAAAAAGGTGGGGTTACTCTTTACATTTCATGGGTTATGTAATTGGATG7660.15166394753932175No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7590.15027798457225222No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7560.14968400044350816No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7410.14671407979978773No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA7410.14671407979978773No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7310.14473413270397414No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCATCCCAAAAGGCCTCCGT6950.1376063231590452No Hit
ACCTCGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6870.13602236548239433No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACC6800.13463640251532477No Hit
GCACGGGACCATGCAGGACCTGCACGACTCCTGCTCAAGGAACCTCTATG6750.133646428967418No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCC6660.13186447658118577No Hit
GTTGTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGTGC6470.1281025770991399No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6290.12453867232667543No Hit
CAAAAGATACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT6280.12434067761709408No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT6190.12255872523086182No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCAAATTCCAGGATCTTCAACCACC5960.11800484691049054No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGGGAGTCCAA5950.1178068522009092No Hit
GTATCTTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGAGATAC5880.11642088923383967No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5750.11384695800928199No Hit
ACAAAAGATACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGA5670.11226300033263112No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5650.11186701091346839No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCACAGAATGAGGCGCTATGTGTTGTTTCTCTCTTATATAAGATAC5640.11166901620388704No Hit
CACCACTTCTTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCCCTGT5560.11008505852723616No Hit
ATACTGCACTCAGGCAAGCAGTTCTTTGCTGGGGGGATCTAATAAATCTA5510.10909508497932935No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5510.10909508497932935No Hit
CATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5420.10731313259309712No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTTGAGGACAAACGGGCAAC5420.10731313259309712No Hit
CTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTAT5370.10632315904519031No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5250.10394722253021399No Hit
GTTCTGCGCAAGGTCCCCAATCCTCGAGAAGATTGACGATATGTGAGAGA5240.10374922782063263No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGGA5160.10216527014398176No Hit
GCCTTAGAGTCTTCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5130.10157128601523766No Hit
GTCCAACATCTTGAGTCACTTTTTACCGCTGTTACCAATTTTCTTTTGTC5100.10097730188649359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)