FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493096
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA27810.5639875399516524No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC27240.5524279247854373No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC25270.5124762723688694No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17470.354292064831189No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG16560.3358372406184597No Hit
CCTCCAGCTTATAGACCAGTAAATGCCCCTATCTTATCAACACTTCCGGA14230.28858477862322957No Hit
GTATGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGACTACTG13630.2764167626587926No Hit
CTCTAGGACTATATCTCTTCCAAAAGTGAGACAAGAAACGTGAAACCACA12980.26323474536398594No Hit
GTGTTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG11680.23687071077437252No Hit
GTACCAAACCTTTGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC11330.22977270146178433No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT11110.22531109560815746No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10990.22287749241527002No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA10660.21618508363482974No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10590.21476548177231208No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG10550.2139542807080163No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10050.20381426740431882No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCC9970.20219186527572724No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9890.20056946314713567No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA9660.1959050570274348No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC9510.1928630530363256No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9450.1916462514398819No Hit
GCATACAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGT8970.18191183866833233No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8930.18110063760403652No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8790.17826143387900123No Hit
GGTCTTAGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCC8730.17704463228255754No Hit
CATTTAAACCCTAACAAAACTAAACGATGGGGTTACTCTTTAAATTTCAT8650.17542223015396596No Hit
GGGGAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT8350.1693382221717475No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8300.16832422084137774No Hit
TCTGTGGAGTTACTCTCATTTTTGCCTTCCGACTTCTATCCTTCGGTACG8240.16710741924493405No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC8100.16426821551989876No Hit
GGTGTTTGAAGATCCTGGAATTAGAGGACAGACGGGCAACATACCTTGAT8090.16406541525382481No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT8010.1624430131252332No Hit
ATATTAGTGTTGACATAGCTGACTACTAGGTCCCTAGATGTTGGATCTTC8010.1624430131252332No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC7960.1614290117948635No Hit
CCAGCAGGAAAATACAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTG7930.16082061099664163No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7790.15798140727160634No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7790.15798140727160634No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7660.155345003812645No Hit
GTATCGGGATGCCTTAGAATCTCCTGAGCATTGTACACCTCACCATACTG7640.1549394032804971No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7590.15392540195012736No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA7230.1466245923714652No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7060.14317698784820806No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6900.13993218359102488No Hit
GTATGGATCGGTAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6820.13830978146243328No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT6710.13607897853561984No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGG6590.13364537534273244No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT6400.12979217028732742No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6390.12958937002125345No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6360.12898096922303162No Hit
GTTTTGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGAAGTC6290.127561367360514No Hit
CACTAATATGGGTCTAAAGTTCAGGCAACTATTGTGGTTTCACGTTTCTT6280.12735856709444004No Hit
CCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTC6280.12735856709444004No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC6220.12614176549799633No Hit
CCAGCATACAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAAGT6180.12533056443370053No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC6140.12451936336940475No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT6130.1243165631033308No Hit
CCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTT6130.1243165631033308No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6070.1230997615068871No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC6060.12289696124081315No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAGGA6050.1226941609747392No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6020.12208576017651736No Hit
TCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGGAACTAATGA5970.1210717588461476No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5920.12005775751577785No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5890.11944935671755602No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5890.11944935671755602No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG5740.11640735272644678No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATTC5640.11437935006570728No Hit
GTACAAAACCTTTGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5630.11417654979963333No Hit
AACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGC5610.11377094926748545No Hit
GTTTTTTCCTGGAATTAGAGGACAGACGGGCAACATACCTTGATAGTCCA5560.11275694793711569No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA5460.11072894527637621No Hit
TTGTATGCCTGTATTCAATCGAAGCAGGCTTTCACTTTCTCGCCAACTTA5420.10991774421208042No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5420.10991774421208042No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5410.10971494394600646No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5360.1087009426156367No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG5360.1087009426156367No Hit
CTTTGGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGGA5330.10809254181741487No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5280.10707854048704513No Hit
CCACCAAACTCTGCAAGATCCCAGGGTGAGAGGCCTGTATTTTCCTGCTG5260.10667293995489723No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5260.10667293995489723No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATC5230.10606453915667537No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5190.10525333809237958No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCGAAGC5180.10505053782630562No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT5150.10444213702808379No Hit
GTAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACG5100.10342813569771403No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5050.10241413436734428No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5050.10241413436734428No Hit
GTAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAG4980.10099453250482665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)