FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493096
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA28650.5810227623018641No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23830.4832730340542207No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC23090.46826581436474846No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG19110.3875513084673167No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15830.3210328211950614No Hit
CCTCCAGCTTATAGACCAGTAAATGCCCCTATCTTATCAACACTTCCGGA14280.2895987799535993No Hit
GTATGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGACTACTG13690.2776335642552363No Hit
CTCTAGGACTATATCTCTTCCAAAAGTGAGACAAGAAACGTGAAACCACA12100.2453883219494784No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA11490.2330175057189675No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG11330.22977270146178433No Hit
GTACCAAACCTTTGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC10960.22226909161704822No Hit
GTGTTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG10750.21801028602949526No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10400.21091227671690707No Hit
GGGGAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT10280.20847867352401966No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT10000.2028002660739491No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9750.19773025942210037No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC9330.1892126482469945No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9260.18779304638447686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8770.17785583334685334No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA8540.17319142722715253No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8480.17197462563070884No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCC8390.1701494232360433No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT8270.16771582004315588No Hit
GGTCTTAGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCC8250.167310219511008No Hit
GCATACAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGT8070.1636598147216769No Hit
CATTTAAACCCTAACAAAACTAAACGATGGGGTTACTCTTTAAATTTCAT8060.16345701445560296No Hit
CCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTT7910.16041501046449375No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7760.1573730064733845No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7590.15392540195012736No Hit
ATATTAGTGTTGACATAGCTGACTACTAGGTCCCTAGATGTTGGATCTTC7580.1537226016840534No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7560.15331700115190552No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7550.15311420088583155No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7540.1529114006197576No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7520.15250580008760972No Hit
TCTGTGGAGTTACTCTCATTTTTGCCTTCCGACTTCTATCCTTCGGTACG7300.14804419423398285No Hit
GTATCGGGATGCCTTAGAATCTCCTGAGCATTGTACACCTCACCATACTG7130.1445965897107257No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC7110.14419098917857778No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7100.14398818891250387No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7050.1429741875821341No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC6880.13952658305887697No Hit
CTTTGGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGGA6790.13770138066421142No Hit
CCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTC6780.13749858039813748No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6780.13749858039813748No Hit
CCAGCAGGAAAATACAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTG6740.13668737933384167No Hit
GTATGGATCGGTAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6700.13587617826954587No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT6580.1334425750766585No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6580.1334425750766585No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6530.13242857374628875No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC6470.13121177214984506No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6400.12979217028732742No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC6380.1293865697551795No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGG6320.12816976815873582No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6270.12715576682836607No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG6180.12533056443370053No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC6050.1226941609747392No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5990.1214773593782955No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAGGA5970.1210717588461476No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT5940.12046335804792577No Hit
CACTAATATGGGTCTAAAGTTCAGGCAACTATTGTGGTTTCACGTTTCTT5910.11985495724970391No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG5810.11782695458896443No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCGAAGC5680.1151905511300031No Hit
AACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGC5600.11356814900141149No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5560.11275694793711569No Hit
GGTGTTTGAAGATCCTGGAATTAGAGGACAGACGGGCAACATACCTTGAT5550.11255414767104176No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5500.111540146340672No Hit
CCAGCATACAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAAGT5480.11113454580852411No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATC5470.11093174554245015No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT5450.11052614501030225No Hit
TCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGGAACTAATGA5370.10890374288171066No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5250.10647013968882327No Hit
TTGTATGCCTGTATTCAATCGAAGCAGGCTTTCACTTTCTCGCCAACTTA5230.10606453915667537No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5230.10606453915667537No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5130.10403653649593589No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5120.10383373622986193No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTT5030.10200853383519638No Hit
GTAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACG4970.1007917322387527No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4960.10058893197267874No Hit
GTACAAAACCTTTGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4950.10038613170660479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)