FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403134
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21060.5224069416124663No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14530.36042606180575193No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11330.2810479890061369No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10070.2497928728412885No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9380.2326769758938715No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAATGCTTCCGGA9250.22945224168638717No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT9070.2249872250914088No Hit
GTACAACACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8960.22225860383892204No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT8400.20836744109898941No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8090.20067769029652674No Hit
CTCTAAGACCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7820.19398016540405918No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA7470.1852981886916013No Hit
ACGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT6620.16421338810420355No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6430.15950031503172643No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATTC6370.15801197616673365No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT6270.15553141139174567No Hit
AATACACGTCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6070.15057028184176974No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5900.14635332172429016No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5790.14362470047180342No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT5750.14263247456180822No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5410.13419855432684913No Hit
GGTTAGGAAGTAATGATTAACTAGATTTTCTGGATAATAAGGTTTAATAC5380.13345438489435277No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5370.13320632841685395No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5300.1314699330743624No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5240.1299815942093696No Hit
CCTTTGAGAAATACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTT5220.129485481254372No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG5220.129485481254372No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG5220.129485481254372No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5200.1289893682993744No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA5190.1287413118218756No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5190.1287413118218756No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5110.1267568600018852No Hit
GGGTAAAGGTGCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5080.12601269056938885No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTTACAC5030.12477240818189485No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA4970.12328406931690207No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4950.12278795636190447No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTGATGGCTTCTTTCCTTCCGTACG4780.11857099624442494No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT4720.11708265737943216No Hit
GTGTAAACAATACCTGCACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4650.11534626203694057No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4650.11534626203694057No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4600.11410597964944659No Hit
GGTTTGGAAGTAATGATTAACTAGATTTTCTGGATAATAAGGTTTAATAC4560.1131137537394514No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTG4500.11162541487445862No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4470.11088124544196222No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4410.10939290657696944No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCGGCCTCTACCAATCGCCAGTC4400.10914485009947066No Hit
CCACCAAACTCTGCAAGATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTG4280.10616817236948509No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4250.10542400293698868No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4230.1049278899819911No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4220.10467983350449231No Hit
ATCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4190.1039356640719959No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAAAATCTAGTTAATCATTACT4120.10219926872950434No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4080.10120704281950915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)