FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565336
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA27100.4793609464106301No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24300.42983287814680116No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG21740.38455007287701476No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC21390.3783590643440361No Hit
AACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGAC19670.3479346795533983No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17040.3014136725770162No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC14670.25949170051084663No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14470.25595398134914454No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG12880.22782911401361314No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCGCTACCAATCGCCAGTC12740.2253527106004217No Hit
CTCACTGTTAGTATTCCTTGGACGCATAAGGTGGGAAACTTTACGGGGCT12440.2200461318578686No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11530.2039495096721242No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11430.20218065009127315No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT10990.1943976679355286No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT10680.18891420323489042No Hit
CTCTAAGACCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10590.18732222961212447No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10440.18466894024084793No Hit
CCTTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10090.1784779317078693No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCGTC10090.1784779317078693No Hit
TCCAACGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGATTG9950.17600152829467786No Hit
CTCTAGCCACCTGGGTGGGTGGTAATTTGGACGATCCAACATCCAGGGAC9830.1738788967976566No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9760.1726406950910609No Hit
ATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTG9720.1719331512587205No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9290.16432705506106104No Hit
GTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAG8990.15902047631850794No Hit
GGATAAAGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8920.1577822746119122No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8870.1568978448214867No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8840.15636718694723137No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8730.15442144140829525No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA8490.15017617841425276No Hit
GTTTAATACCTTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8430.14911486266574214No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA8430.14911486266574214No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT8170.14451582775552946No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG7870.13920924901297635No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7720.1365559596416998No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7620.13478710006084876No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7620.13478710006084876No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7540.13337201239616794No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT7520.13301824047999775No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7490.13248758260574242No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7420.1312493808991467No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7360.13018806515063608No Hit
ATCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC7330.12965740727638078No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7280.12877297748595526No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA7240.12806543365361484No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGCGTCCAAGGAAT7230.12788854769552974No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT7070.1250583723661681No Hit
GAGAAACGGTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACT7060.12488148640808298No Hit
GTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCAGGATTCAGCGCCGACGGG7020.12417394257574256No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG6970.12328951278531705No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6960.12311262682723194No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6930.12258196895297664No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6900.12205131107872133No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT6820.12063622341404051No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC6810.12045933745595541No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT6800.1202824514978703No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6740.11922113574935966No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6600.11674473233616824No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6540.1156834165876576No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6510.11515275871340229No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6500.11497587275531719No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6480.11462210083914698No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA6460.11426832892297678No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6420.11356078509063637No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6340.11214569742595555No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG6320.11179192550978534No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6290.11126126763553001No Hit
CCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTGC6230.11019995188701939No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6160.10896175018042369No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6120.10825420634808328No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6100.10790043443191306No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT6080.10754666251574287No Hit
GCGTCTGGCCAGGTGTCCTTGTTGGGATTGAAGTCCCAATCTGGATTTGC6060.10719289059957264No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6060.10719289059957264No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6030.10666223272531733No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6020.10648534676723223No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA5970.10560091697680671No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5960.10542403101872161No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5940.10507025910255141No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5880.10400894335404079No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5820.10294762760553015No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5800.10259385568935994No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5770.10206319781510464No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGT5770.10206319781510464No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5740.10153253994084933No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5720.10117876802467912No Hit
GCTTGGGACTGTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCAACCACG5700.10082499610850892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)