FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329273
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA20150.6119542142841958No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC19220.5837101736249253No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC16910.5135556210196403No Hit
TAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG16280.49442256121819894No Hit
GTGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGC13230.4017942558302685No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12280.37294281644714267No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12070.3665651298466622No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11900.36140224069389226No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10500.3188843300240226No Hit
CTCTAAGACTGTTTCTCTTCCAAAAGAGAGGCAAGAAATGTGAAACCACA9250.2809219097830675No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8950.27181092892523834No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCCGCCTCTACCAATCGCCAGTC8920.2708998308394554No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8840.2684702359440343No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8800.2672554384963237No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT8660.2630036474293368No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC8620.26178884998162616No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8190.24872977741873764No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACT7790.236581802941632No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC7390.22443382846452636No Hit
CTCTTGTACATGTCCTACTATTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7150.21714504377826302No Hit
CTTTTACACAGTGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7070.2147154488828419No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6620.20104897759609808No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6560.19922678142453223No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6540.19861938270067692No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6470.19649348716718348No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6460.19618978780525584No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6430.1952786897194729No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6430.1952786897194729No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC6410.1946712909956176No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6360.19315279418597942No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6260.190115800566703No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6150.18677510758549895No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6150.18677510758549895No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6150.18677510758549895No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5770.17523453183224863No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5710.1734123356606828No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5550.16855314586984052No Hit
GCCTAATCATCTCTTGTACATGTCCTACTATTCAAGCCTCCAAGCTGTGC5530.16794574714598523No Hit
CCTTTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5460.16581985161249177No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5460.16581985161249177No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA5450.16551615225056412No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5410.16430135480285354No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5410.16430135480285354No Hit
GATATGATGTATATTTGCGGGAAAGAACAACAGAGTTATCTGTCCCGAGA5270.1600495637358666No Hit
GCCTAGCAGCCATGGATATGATGTATATTTGCGGGAAAGAACAACAGAGT5230.15883476628815604No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5220.1585310669262284No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAA5190.15761996884044546No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5190.15761996884044546No Hit
GGATAAGGGTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACT5080.15427927585924142No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5080.15427927585924142No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5050.1533681777734585No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC4910.14911638670647154No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4890.14850898798261625No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4800.1457756937252675No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC4790.14547199436333982No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4760.14456089627755692No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC4760.14456089627755692No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4740.14395349755370163No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4740.14395349755370163No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4700.14273870010599107No Hit
CTTGTACATGTCCTACTATTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4690.14243500074406343No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4650.14122020329635288No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGT4610.14000540584864232No Hit
GTTATTAGACGACGAGTCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG4600.13970170648671468No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4480.13605731414358296No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4400.13362771924816186No Hit
TCCTAATACAAAGACCTTTAACCTGATCTCCTCCCCCAACTCCTCCCAGT4400.13362771924816186No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4380.1330203205243066No Hit
CTTCTAGATACCGCCTCAGCTCTGTTCCGGGAAGCTTTAGAATCTCCTGA4350.13210922243852366No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4330.1315018237146684No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCATCAATCTTCTCG4330.1315018237146684No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4330.1315018237146684No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4260.12937592818117488No Hit
GTACATGTCCTACTATTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4190.12725003264768142No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4170.12664263392382613No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4150.12603523519997084No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG4120.12512413711418793No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4110.12482043775226027No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGTCCGAATGCTCCAG4100.12451673839033264No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4090.124213039028405No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAATTAATGA4080.12390933966647737No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4060.12330194094262208No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4020.12208714349491152No Hit
GGATAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3990.12117604540912859No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3980.12087234604720096No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3940.11965754859949039No Hit
CCTGTATTCCCATCCCACCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3920.11905014987563511No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3920.11905014987563511No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3910.11874645051370748No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT3900.11844275115177982No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3900.11844275115177982No Hit
GACAGGTACAGTGGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAG3890.11813905178985218No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCCACTG3850.11692425434214161No Hit
CCTCACCATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAATTAAT3850.11692425434214161No Hit
CATCATATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG3800.11540575753250341No Hit
ATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAATTAATGACTCTA3800.11540575753250341No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA3670.1114576658274441No Hit
TCTAGATACCGCCTCAGCTCTGTTCCGGGAAGCTTTAGAATCTCCTGAGC3670.1114576658274441No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3640.11054656774166116No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA3600.10933177029395062No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3570.10842067220816769No Hit
CTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCCTC3560.10811697284624006No Hit
CCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT3550.10781327348431241No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3530.10720587476045712No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT3530.10720587476045712No Hit
ATGTATATTTGCGGGAAAGAACAACAGAGTTATCTGTCCCGAGAATGTTT3500.10629477667467421No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3470.10538367858889128No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCACCATCC3450.10477627986503601No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT3450.10477627986503601No Hit
CCATATTCTTGGGAACAAGAGATACAGCATGGGGCAGAATCTTTCCACCA3430.10416888114118071No Hit
GTTCTGGATAGTAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA3410.10356148241732543No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT3390.10295408369347017No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3390.10295408369347017No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3380.10265038433154251No Hit
GCCATGGATATGATGTATATTTGCGGGAAAGAACAACAGAGTTATCTGTC3370.10234668496961487No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3360.10204298560768724No Hit
ATATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC3360.10204298560768724No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG3360.10204298560768724No Hit
CACCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3350.1017392862457596No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC3310.10052448879804904No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3300.1002207894361214No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3300.1002207894361214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)