FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322067
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC15530.4821978035626127No Hit
TACCCTGATAGTCCAGTAGAACCAACAAGAAGATGAGGCATAGCAGCAGG15490.48095582596167874No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG12490.3878075058916312No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA12390.38470256188929636No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12200.37880316828486No Hit
ATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATCTTCAACCACC11400.35396361626618067No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10540.32726109784610036No Hit
TACCTTGATAGTCCAGTAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9760.303042534627888No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA9370.2909332530187818No Hit
ATCAGGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATCTTCAACCACC8850.27478754420664026No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA8650.26857765620197044No Hit
CATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6850.21268866415994186No Hit
ATGTAAGACCTTGGGCAAGACGTGGTGGGCGTTCACGGTGGTCTCCATGC6560.2036843265531706No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6560.2036843265531706No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT6360.19747443854850077No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6280.19499048334663285No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG6200.1925065281447649No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACC6140.19064356174336394No Hit
GTGCTGGGCTGAGCATTAAATATCACCCTCTATGATTAATTGGAAGTTAA6100.18940158414243No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6090.18909108974219652No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACGTGGTGGGCGTTC5880.18257070733729316No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5780.17946576333495826No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5750.1785342801342578No Hit
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAAA5630.1748083473314559No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5470.16984043692772002No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5370.1667354929253851No Hit
GTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5360.1664249985251516No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA5230.1623885713221162No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5160.16021511052048176No Hit
GTCTAGTTTGGAAGTAATGATTAACTAAATGTTCCGGATAATAAGGTTTA5000.1552472001167459No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4940.15338423371534493No Hit
GAGCGTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCC4810.14934780651230953No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4690.14562187370950763No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT4630.1437589073081067No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4600.14282742410740623No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACG4560.14158544650647226No Hit
ATATACATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC4440.13785951370367036No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4420.13723852490320337No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC4340.13475456970133542No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4100.12730270409573163No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG4090.12699220969549815No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACACACGGGCAAC4080.12668171529526465No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3980.12357677129292972No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA3970.12326627689269624No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG3910.12140331049129528No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCAAGGA3910.12140331049129528No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3890.1207823216908283No Hit
GTACAGTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAA3860.11985083849012781No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG3840.11922984968966086No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG3760.1167458944877929No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT3740.11612490568732592No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA3720.11550391688685896No Hit
GTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAAT3690.11457243368615848No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG3660.113640950485458No Hit
CTCAAGCTCAGGGCATAATGCAAACCCTGCCAGCAAATCCGCCTCCTGCA3660.113640950485458No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3620.11239897288452402No Hit
ACGACATCTTCTAGATACCGCCTCAGCTCTGTACCGGGATGCCTTAGAGT3600.11177798408405705No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA3590.11146748968382356No Hit
AAACCAGACATTATTTACACACTCTATGGAAGGCGGGTATATTATATAAG3550.11022551208288958No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3540.10991501768265609No Hit
GTTAATAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT3530.1096045232824226No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3520.1092940288821891No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3510.10898353448195561No Hit
GTCTTGGTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT3460.10743106248078817No Hit
CTCACGCTCAGGGCATAATACAAACCCTGCCAGCAAATCCGCCTCCTGCA3410.1058785904796207No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT3400.1055680960793872No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3320.10308414087751927No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3320.10308414087751927No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG3300.10246315207705228No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3280.1018421632765853No Hit
GGTAGATGCAGGAGGCGGATTTGCTGGCAGGGTTTGTATTATGCCCTGAG3260.10122117447611834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)