FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322067
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC15700.4874762083665821No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG14990.46543110595000414No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA12980.40302173150307236No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11890.36917784187762176No Hit
TACCCTGATAGTCCAGTAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11560.3589315266699165No Hit
ATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATCTTCAACCACC11060.3434068066582419No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9260.2875178146162134No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA9140.2837918818134115No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA8080.25087947538866134No Hit
ATCAGGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATCTTCAACCACC7860.24404859858352454No Hit
TACCTTGATAGTCCAGTAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7280.22603992336998202No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6980.2167250913629773No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6680.20741025935597251No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG6650.20647877615527205No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6510.20213185455200317No Hit
GTCTAGTTTGGAAGTAATGATTAACTAAATGTTCCGGATAATAAGGTTTA6160.19126455054383093No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACC6140.19064356174336394No Hit
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAAA5920.18381268493822714No Hit
GTGCTGGGCTGAGCATTAAATATCACCCTCTATGATTAATTGGAAGTTAA5760.17884477453449127No Hit
CATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5660.17573983053215636No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5240.1626990657223497No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT5210.16176758252164922No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5120.1589731329195478No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5060.15711016651814683No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4940.15338423371534493No Hit
GAGCGTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCC4800.14903731211207608No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC4660.1446903905088072No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4650.14437989610857369No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4640.1440694017083402No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT4630.1437589073081067No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT4550.14127495210623878No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4540.14096445770600527No Hit
GTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4530.1406539633057718No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4480.1391014913046043No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCAAGGA4460.13848050250413732No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4360.1353755585018024No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4340.13475456970133542No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4240.13164962569900052No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACACACGGGCAAC4190.13009715369783306No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACGTGGTGGGCGTTC4160.1291656704971326No Hit
GTACAGTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAA4140.1285446816966656No Hit
ATGTAAGACCTTGGGCAAGACGTGGTGGGCGTTCACGGTGGTCTCCATGC4100.12730270409573163No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4020.1248187488938637No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4000.12419776009339671No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3860.11985083849012781No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC3850.11954034408989435No Hit
GTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAAT3820.11860886088919387No Hit
CTCACGCTCAGGGCATAATACAAACCCTGCCAGCAAATCCGCCTCCTGCA3730.11581441128709245No Hit
ATATACATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC3690.11457243368615848No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3680.11426193928592498No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA3660.113640950485458No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG3620.11239897288452402No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC3570.11084650088335657No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACG3500.10867304008172211No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG3470.10774155688102166No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3460.10743106248078817No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG3450.10712056808055467No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA3440.10681007368032118No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3400.1055680960793872No Hit
TCTCGGGGCCGCTTGGGGATCTTTCGTCCCCTTCTCCGTCTGCCGTTTCG3360.10432611847845324No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3360.10432611847845324No Hit
GTCTTGGTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT3300.10246315207705228No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3270.1015316688763518No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3240.10060018567565135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)