FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569209
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA33720.5924010337152082No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA28880.5073707548545437No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC28400.49893799992621346No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT16650.2925111865764596No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG16040.2817945605217064No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC15940.2800377365783043No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT15060.2645776858763653No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCGTTGACATACT15020.2638749562990044No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA14760.25930721404615875No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14720.2586044844687979No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT14700.2582531196801175No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA14580.25614493094803487No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA14310.25140150630084906No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG13610.23910373869703394No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG13600.23892805630269373No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA13410.23559009081022964No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACTACAGAAT12310.2162650274328059No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA11950.2099404612365581No Hit
GTATTTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG11610.20396725982899075No Hit
ATGTAAGACCGTTGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC11460.20133202391388752No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11390.200102247153506No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT11360.19957519997048537No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG11290.19834542321010384No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG11220.19711564644972232No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTACGT11140.19571018729500061No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC10970.1927235865912169No Hit
CTACAGAATTGTCAGTCCCGATAAGTTTTGCTCCAGACCGGCTGTGAGCA10940.19219653940819628No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10940.19219653940819628No Hit
ATTCTGTAGTGCTCTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGC10800.18973698588743326No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA10800.18973698588743326No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10790.18956130349309305No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC10720.18833152673271153No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC10630.1867503851836496No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10290.18077718377608223No Hit
CAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG10260.1802501365930616No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT9860.17322284081945297No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGAAAACTTCCTGTAA9830.1726957936364323No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG9800.17216874645341165No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT9730.17093896969303016No Hit
ATGTATACTTGCGGGAGAGCACTACAGAATTGTCAGTCCCGATAAGTTTT9720.17076328729868992No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9720.17076328729868992No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCG9480.16654690983452475No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA9480.16654690983452475No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA9460.16619554504584433No Hit
GTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGA9460.16619554504584433No Hit
GAGCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA9340.16408735631376173No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC9300.16338462673640086No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9270.1628575795533802No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC9260.16268189715904No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9100.15987097884959653No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT9100.15987097884959653No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG9060.1591682492722357No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8970.15758710772317375No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8940.1570600605401531No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATGATTTGATTTTTTGT8900.15635733096279222No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATGCC8850.15547891899109115No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG8790.15442482462504986No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC8700.15284368307598792No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG8690.1526680006816477No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC8640.1517895887099466No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG8620.15143822392126616No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA8610.15126254152692595No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8580.1507354943439053No Hit
GTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8500.14933003518918359No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT8450.1484516232174825No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8380.14722184645710099No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG8270.14528934011935862No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8230.14458661054199776No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT8130.1428297865985956No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA8040.14124864504953366No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8030.14107296265519342No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG7990.14037023307783256No Hit
CTGTATCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTAC7990.14037023307783256No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCATT7950.1396675035004717No Hit
GTTTACAGGAAGTTTTCTAAAACATGATTTGATTTTTTGTACAATATGTT7950.1396675035004717No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC7880.1384377267400902No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7850.13791067955706954No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG7760.1363295380080076No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAAT7690.1350997612476261No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7660.13457271406460544No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC7650.13439703167026523No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC7630.1340456668815848No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTTACTTT7620.13386998448724458No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7560.1328158901212033No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA7470.13123474857214135No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7460.1310590661778011No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG7440.1307077013891207No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7380.12965360702307938No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA7360.12930224223439896No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7310.12842383026269788No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC7300.12824814786835767No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACA7280.12789678307967722No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA7230.12701837110797615No Hit
AGTCTGTACAACATCTTGAATCCCTTTATGCCGCTGTTACCAATTTTCTT7170.12596427674193486No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATT6940.12192358167210988No Hit
GCACTACAGAATTGTCAGTCCCGATAAGTTTTGCTCCAGACCGGCTGTGA6790.11928834575700666No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6760.118761298573986No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA6700.1177072042079447No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6690.11753152181360449No Hit
AGAGCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGG6670.11718015702492407No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6630.1164774274475632No Hit
GCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAAATACTGTT6630.1164774274475632No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC6620.11630174505322297No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGCGGAACCTTTGTGTC6610.11612606265888276No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6580.11559901547586213No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA6560.11524765068718168No Hit
GGGTAAAGGTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6550.11507196829284147No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6520.11454492110982081No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6500.1141935563211404No Hit
GCATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6480.11384219153245997No Hit
CTGTAGTGCTCTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGCTGT6470.11366650913811974No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA6440.1131394619550991No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6420.11278809716641866No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT6360.11173400280037736No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA6330.1112069556173567No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6320.1110312732230165No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6310.11085559082867628No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6260.10997717885697521No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6220.10927444927961434No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA6200.10892308449093392No Hit
CCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCA6130.1076933077305524No Hit
CATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGC6120.10751762533621217No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6110.10734194294187197No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT6090.10699057815319153No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6090.10699057815319153No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAG6060.10646353097017089No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAAATACTGTTTACTT5970.10488238942110895No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5960.10470670702676874No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5940.10435534223808829No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC5920.10400397744940787No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5910.10382829505506766No Hit
ACACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG5850.10277420068902635No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5840.10259851829468614No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG5830.10242283590034593No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5820.1022471535060057No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA5800.10189578871732527No Hit
ATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC5750.10101737674562418No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGATTC5730.10066601195694376No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA5700.1001389647739231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)