FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569209
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA27270.47908588936576896No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA27240.4785588421827483No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC22000.38650126754847514No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG16430.28864617390097486No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG16350.2872407147462531No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT15100.26528041545372616No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA13990.24577966968196216No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCGTTGACATACT13960.2452526224989415No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA13460.2364685027819307No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT13380.23506304362720898No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC13130.23067098376870357No Hit
GTATTTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG13060.2294412070083221No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA12890.2264546063045384No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT12600.22135981686867215No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA12390.21767048658752763No Hit
ATTCTGTAGTGCTCTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGC11940.2097647788422179No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11520.2023861182799288No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11270.19799405842142342No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACTACAGAAT11140.19571018729500061No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTACGT10690.1878044795496909No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG10510.18464219645156701No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGAAAACTTCCTGTAA10310.18112854856476268No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG10240.17989877180438116No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA10230.17972308941004095No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10030.17620944152323664No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT10000.17568239434021599No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9920.17427693518549425No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9850.17304715842511276No Hit
CTACAGAATTGTCAGTCCCGATAAGTTTTGCTCCAGACCGGCTGTGAGCA9830.1726957936364323No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9760.1714660168760508No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC9640.16935782814396821No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT9420.16549281546848346No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC9410.16531713307414325No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG9250.16250621476469979No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9210.16180348518733892No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA9120.16022234363827698No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9020.15846551969487482No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA8980.15776279011751396No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8900.15635733096279222No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8890.15618164856845201No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC8860.15565460138543136No Hit
CAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG8790.15442482462504986No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8790.15442482462504986No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC8750.153722095047689No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8700.15284368307598792No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8670.15231663589296726No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG8650.15196527110428681No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG8550.15020844716088466No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATGATTTGATTTTTTGT8420.14792457603446185No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8390.14739752885144122No Hit
ATGTATACTTGCGGGAGAGCACTACAGAATTGTCAGTCCCGATAAGTTTT8310.1459920696967195No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG8260.1451136577250184No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT8190.1438838809646369No Hit
GAGCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA8180.14370819857029668No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG8080.14195137462689453No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG8040.14124864504953366No Hit
GTTTACAGGAAGTTTTCTAAAACATGATTTGATTTTTTGTACAATATGTT8030.14107296265519342No Hit
GTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8000.14054591547217277No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATGCC8000.14054591547217277No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT8000.14054591547217277No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7980.14019455068349235No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT7910.13896477392311082No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7900.13878909152877061No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG7820.1373836323740489No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7800.13703226758536846No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7700.1352754436419663No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA7680.1349240788532859No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGCGGAACCTTTGTGTC7630.1340456668815848No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCG7630.1340456668815848No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7570.1329915725155435No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7550.13264020772686308No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7490.13158611336082177No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA7450.1308833837834609No Hit
ATGTAAGACCGTTGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC7440.1307077013891207No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC7430.13053201899478048No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGATTC7380.12965360702307938No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACA7320.1285995126570381No Hit
CTGTAGTGCTCTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGCTGT7280.12789678307967722No Hit
GTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC7220.12684268871363594No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG7160.12578859434759465No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7150.12561291195325444No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7070.12420745279853271No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC6990.12280199364381097No Hit
CTGTATCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTAC6940.12192358167210988No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG6940.12192358167210988No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTTACTTT6900.12122085209474902No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6780.11911266336266645No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6760.118761298573986No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6720.11805856899662515No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG6710.11788288660228492No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6680.11735583941926428No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6650.11682879223624362No Hit
AGTCTGTACAACATCTTGAATCCCTTTATGCCGCTGTTACCAATTTTCTT6610.11612606265888276No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC6530.11472060350416105No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAAT6520.11454492110982081No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6430.11296377956075888No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT6300.11067990843433607No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6270.11015286125131542No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6240.10962581406829479No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATT6220.10927444927961434No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6140.10786899012489261No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG6130.1076933077305524No Hit
AGAGCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGG6090.10699057815319153No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6080.10681489575885131No Hit
GTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGA6070.1066392133645111No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6000.1054094366041296No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6000.1054094366041296No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA5980.10505807181544916No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC5980.10505807181544916No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5960.10470670702676874No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5960.10470670702676874No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5950.1045310246324285No Hit
GGGTAAAGGTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5930.10417965984374808No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5910.10382829505506766No Hit
GCACTACAGAATTGTCAGTCCCGATAAGTTTTGCTCCAGACCGGCTGTGA5850.10277420068902635No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5830.10242283590034593No Hit
GCATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC5800.10189578871732527No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA5800.10189578871732527No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG5790.10172010632298505No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5770.10136874153430463No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5730.10066601195694376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)