FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467246
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCCACACTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG27110.5802082842870779No Hit
GATTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG24380.5217808178133146No Hit
GTGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC20830.44580370939505104No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG19950.42696994730827015No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18260.39080056330070245No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17830.3815977022810254No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG16790.35933961981482987No Hit
ATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA16010.3426460579651832No Hit
CCTTCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15970.34178997787032955No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG15490.3315170167320855No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG14500.310329034384457No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC14280.3056205938627618No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14120.3021962734833471No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT13990.29941401317507266No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12940.27694191068516366No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA11930.25532588829010844No Hit
GAAAAGATGTATACTTGCGGGAGAGCACGACAGAATTGTCCGTCCCGATG11550.2471931273889985No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA11090.23734820629818126No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC10940.23413790594248No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10930.23392388591876653No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACCAAACC10600.22686122513622375No Hit
GGGCGAAATCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAAT10390.22236680463824193No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10380.22215278461452853No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10310.22065464444853458No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9870.2112377634051442No Hit
ACCTCATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTG9820.2101676632865771No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9530.20396108259888795No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9430.20182088236175375No Hit
GCACAACTTCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACC8760.1874815407729547No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8620.18448526044096686No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT8500.18191702015640585No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8330.1782786797532777No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAAT8330.1782786797532777No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG8280.17720857963471062No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCCGTCCCGATG8040.17207209906558857No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7970.17057395889959465No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCCGTCCCGATGAGTTT7760.16607953840161285No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7640.16351129811705184No Hit
GTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAG7610.16286923804591158No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7570.1620131579510579No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7550.16158511790363106No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7440.15923089764278345No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7390.15816079752421636No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTATAAGTCC7130.15259627690766747No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7000.14981401659939303No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6890.14745979633854545No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6860.14681773626740519No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6860.14681773626740519No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG6830.14617567619626493No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC6820.1459616561725515No Hit
TGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6790.14531959610141124No Hit
ATCTCAATGTTAGTATTCCCTGGACTCATAAGGTGGGAAACTTTACGGGG6780.14510557607769783No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6650.1423233157694234No Hit
TCCAAAGAGTGTGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA6550.1401831155322892No Hit
CATCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG6370.13633075510544765No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6330.135474675010594No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6190.13247839467860614No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA6160.13183633460746585No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA6160.13183633460746585No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACACTGCGTGAAA6120.13098025451261222No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6120.13098025451261222No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6120.13098025451261222No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6010.1286260342517646No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGTCCGAATGCTCCCA5910.12648583401463043No Hit
TCCCTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5910.12648583401463043No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5890.12605779396720357No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5860.1254157338960633No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG5860.1254157338960633No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5860.1254157338960633No Hit
ATCCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5850.1252017138723499No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5790.12391759373006937No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5730.12263347358778887No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5720.12241945356407545No Hit
GTATCTGAACCTCTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5710.12220543354036205No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5690.12177739349293519No Hit
CCCTTACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGG5620.12027925332694127No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTATGGA5590.11963719325580101No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5580.11942317323208758No Hit
GTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTG5530.1183530731135205No Hit
CCTCTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5490.11749699301866683No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTTGT5470.11706895297124No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5460.11685493294752657No Hit
CCATACGGCACTCAGGCAAGCTATCCTGTGTTGGGGTGAGTTGATGAATC5460.11685493294752657No Hit
GGGTAGAGGTTCAGATACTGTTTACTTAGAAAGGACTTATAAGTCGGCGA5450.11664091292381315No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5450.11664091292381315No Hit
GTACAACAGACGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5430.11621287287638632No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5420.11599885285267289No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5400.11557081280524606No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGTTCCGGTGGGCGTTC5380.11514277275781921No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC5360.11471473271039238No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5350.11450071268667897No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5290.11321659254439845No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5250.11236051244954477No Hit
CCTTATGAGTCCAGGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5220.11171845237840453No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT5220.11171845237840453No Hit
GGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG5190.11107639230726427No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5120.10957825214127034No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATT5110.10936423211755691No Hit
GTCCAGGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5090.10893619207013008No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACCAAACCTACGGACGGAAACTGC5040.10786609195156298No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5010.10722403188042273No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5010.10722403188042273No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4970.10636795178556906No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAACAAG4950.10593991173814223No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA4930.1055118716907154No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4860.10401373152472146No Hit
GGATATCCACACTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4840.10358569147729461No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTGGTACAGCAACATGAGGGA4840.10358569147729461No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4840.10358569147729461No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4810.10294363140615435No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAGAGGTTCAGATACTGTTTACTT4800.10272961138244094No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC4780.10230157133501411No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC4770.10208755131130068No Hit
AGACAAGACATTATTTACACACTCTTTGGAAGGCGGGTATCTTATATAAA4770.10208755131130068No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4770.10208755131130068No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATTCT4760.10187353128758725No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4740.10144549124016043No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG4730.101231471216447No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4720.1010174511927336No Hit
GACACTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTT4720.1010174511927336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)