FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467246
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCCACACTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG25300.5414706599949491No Hit
GATTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG21490.4599290309601366No Hit
GTGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC20090.42996622764025805No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG17320.37068268107164104No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15850.3392217375857685No Hit
ATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA15840.3390077175620551No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG14830.31739169516699983No Hit
CCTTCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14750.31567953497729245No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG14670.31396737478758513No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT13900.2974878329616519No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC13270.2840045714677065No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12880.2756577905428832No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG12770.27330357028203556No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12610.2698792499026209No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11660.24954734764984612No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA11380.24355478698587038No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA11280.24141458674873623No Hit
GAAAAGATGTATACTTGCGGGAGAGCACGACAGAATTGTCCGTCCCGATG10750.23007152549192503No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC10580.2264331850887969No Hit
GGGCGAAATCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAAT10570.2262191650650835No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10110.21637424397426622No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9960.21316394361856494No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9870.2112377634051442No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG9620.2058872628123087No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9560.20460314267002822No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACCAAACC9320.19946666210090613No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9060.19390214148435728No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT8960.1917619412472231No Hit
GCACAACTTCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACC8490.18170300013269242No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCCGTCCCGATG8340.17849269977699114No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAAT8320.17806465972956428No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8080.17292817916044226No Hit
CATCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG8080.17292817916044226No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC8060.17250013911301543No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8050.172286119089302No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCCGTCCCGATGAGTTT7620.163083258069625No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7610.16286923804591158No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7530.16115707785620423No Hit
GTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAG7450.15944491766649688No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7370.15773275747678953No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7300.1562346173107956No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7290.15602059728708217No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7210.15430843709737485No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC7020.15024205664681986No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6940.14852989645711254No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTATAAGTCC6800.14553361612512467No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6740.14424949598284414No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6640.14210929574570996No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6530.13975507548486238No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6490.1388989953900087No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGTCCGAATGCTCCCA6480.13868497536629526No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6440.1378288952714416No Hit
TGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6410.13718683520030134No Hit
ATCTCAATGTTAGTATTCCCTGGACTCATAAGGTGGGAAACTTTACGGGG6350.13590271505802082No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG6310.13504663496316716No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6270.13419055486831347No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6240.13354849479717323No Hit
GTACAACAGACGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6170.13205035463117928No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6120.13098025451261222No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACACTGCGTGAAA6070.1299101543940451No Hit
TCCCTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6050.12948211434661827No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6050.12948211434661827No Hit
CCTCTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG6030.1290540742991914No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA6030.1290540742991914No Hit
GTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTG6020.128840054275478No Hit
TCCAAAGAGTGTGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA6000.12841201422805118No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTTGT5970.12776995415691092No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5930.12691387406205723No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA5920.12669985403834383No Hit
GTATCTGAACCTCTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5910.12648583401463043No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5870.12562975391977674No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5850.1252017138723499No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACCAAACCTACGGACGGAAACTGC5830.12477367382492306No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5750.1230615136352157No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5690.12177739349293519No Hit
CCTTATGAGTCCAGGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5630.12049327335065468No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5550.11878111316094733No Hit
GTCCAGGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5530.1183530731135205No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG5450.11664091292381315No Hit
ATCCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5330.11407267263925214No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5310.11364463259182528No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5310.11364463259182528No Hit
GGGTAGAGGTTCAGATACTGTTTACTTAGAAAGGACTTATAAGTCGGCGA5300.11343061256811188No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5290.11321659254439845No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5270.11278855249697163No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5150.11022031221241059No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5100.10915021209384351No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5090.10893619207013008No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5080.10872217204641665No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT5040.10786609195156298No Hit
CCCTTACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGG5040.10786609195156298No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5040.10786609195156298No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5000.1070100118567093No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTATGGA4960.10615393176185564No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4950.10593991173814223No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4860.10401373152472146No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4860.10401373152472146No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4850.10379971150100804No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4790.10251559135872754No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4760.10187353128758725No Hit
GGATATCCACACTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4750.10165951126387385No Hit
GGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG4750.10165951126387385No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGTTCCGGTGGGCGTTC4750.10165951126387385No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG4730.101231471216447No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4710.10080343116902017No Hit
CCATACGGCACTCAGGCAAGCTATCCTGTGTTGGGGTGAGTTGATGAATC4710.10080343116902017No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATTCT4690.10037539112159334No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCAC4680.10016137109787993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)