FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400703
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA29880.7456894507902362No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20790.5188381419654956No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18800.46917542419198255No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT14460.3608657783944717No Hit
CTCTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT11790.2942328857033763No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA11160.27851051776502794No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10670.2662820093685348No Hit
ACATATTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTT9810.24481972932571006No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9770.24382148374232285No Hit
ATATAAGAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9680.24157543117970165No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9050.22585306324135335No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9040.2256035018455065No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9010.22485481765796614No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8650.21587060740748135No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8470.21137850228223898No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8350.20838376553207735No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8310.20738551994869017No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8310.20738551994869017No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7960.1986508710940522No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7680.19166315201034181No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG7640.19066490642695463No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT7570.18891797665602703No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA7320.18267894175985705No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7170.17893552082215505No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACGGAAT7100.17718859105122747No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7040.17569122267614667No Hit
GTTATTAGACGAAGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG7030.17544166128029987No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7010.17494253848860628No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6820.1702008719675171No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6720.1677052580090491No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6680.1667070124256619No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA6650.1659583282381215No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6620.1652096440505811No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6430.1604679775294919No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6420.16021841613364513No Hit
CCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA6420.16021841613364513No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6310.15747324077933034No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAGAGAGAGTCCACACGTAGCGCC6200.15472806542501555No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA6180.15422894263332196No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5980.14923771471638594No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5950.14848903052884554No Hit
GATGTATACTTGCGGGAGAGCACGACGGAATTGTCAGTCCCGATGAGTTT5840.14574385517453076No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC5830.14549429377868395No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5820.14524473238283717No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5770.14399692540360318No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5710.14249955702852235No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5520.13775789050743317No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5490.1370092063198928No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5490.1370092063198928No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5450.1360109607365056No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5360.1337649081738844No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5230.13052061002787602No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5170.12902324165279522No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5150.12852411886110163No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA5070.1265276276943272No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5010.12503025931924644No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4940.12328332954831883No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4930.12303376815247204No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAGC4890.12203552256908484No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4860.12128683838154444No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4850.12103727698569763No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4840.12078771558985082No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCACT4830.12053815419400403No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4820.12028859279815723No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4820.12028859279815723No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4820.12028859279815723No Hit
TCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG4800.11978947000646364No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4730.11804254023553605No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4720.11779297883968926No Hit
AGAACATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTC4700.11729385604799564No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4680.11679473325630205No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACGGAATTGTCAGTCCCGATG4640.11579648767291485No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4450.11105482115182568No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTTC4450.11105482115182568No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4440.11080525975597888No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4410.11005657556843847No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4410.11005657556843847No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4380.10930789138089807No Hit
TCTTTGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCTC4370.10905832998505127No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4370.10905832998505127No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACAGTTTGATTTTTTGT4320.10781052300581728No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA4320.10781052300581728No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG4270.10656271602658327No Hit
GGAACATATTGTACAAAAAATCAAACTGTGTTTTAGAAAACTTCCTGTAA4240.10581403183904288No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA4160.10381754067226849No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT4140.1033184178805749No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT4120.10281929508888128No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGATACAA4110.10256973369303449No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4110.10256973369303449No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4100.10232017229718769No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4060.1013219267138005No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4060.1013219267138005No Hit
CCGCCACTGCTCTGCATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA4060.1013219267138005No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4050.1010723653179537No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG4030.1005732425262601No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4030.1005732425262601No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4010.10007411973456651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)