FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434247
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC34450.7933272999007477No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA26860.6185419818674626No Hit
CCTCCTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCGGA23810.5483054574930858No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC22920.5278102093969561No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG19450.4479017701906979No Hit
CATTTAAACCCTCACAAAACCAAACGATGGGGATACTCCCTTAATTTCAT18740.4315516284510889No Hit
CCTACTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCGGA15610.3594728345849252No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA14300.32930567165691416No Hit
TAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATATCATC13150.30282304771247703No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA12480.28739404071876146No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA12400.2855517712269745No Hit
GTTGTGAGTATGCCCTGAGCCAGAGGGCTCCACCCCAACAAGCCCCCATG12200.28094609749750715No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC12080.2781826932598268No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12040.2772615585139333No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11750.2705833316062057No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT11570.2664382252496851No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA11290.25999028202843083No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT11270.25952971465548413No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC11160.25699659410427705No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTTTG10970.25262120406128313No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9760.22475687799800573No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT8530.19643198456178165No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC8340.1920565945187877No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG8260.19021432502700075No Hit
GTACGGCAGACGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG8210.18906290659463393No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT8050.18537836761106005No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC7930.18261496337337965No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7920.1823846796869063No Hit
TCTATGGAATTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7850.18077269388159273No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT7820.18008184282217263No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA7770.1789304243898058No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7530.173403615914445No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA7470.1720219137956048No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTTT7450.17156134642265808No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7410.1706402116767646No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG7400.17040992799029125No Hit
CCCTTAATTTCATGGGATATGTAATTGGGAGTTGGGGCTCCTTGCCACAG7320.1685676584985043No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7160.16488311951493043No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT7030.16188943159077668No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6800.15659290680188925No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT6760.15567177205599578No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTATGGA6730.15498092099657568No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6730.15498092099657568No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6670.15359921887773548No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC6610.15221751675889528No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6540.15060553095358173No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT6530.15037524726710835No Hit
CCATCGCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAG6460.1487632614617948No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT6400.14738155934295458No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6370.14669070828353448No Hit
ATTTACATACTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACA6300.14507872247822093No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGGTACGG6300.14507872247822093No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6220.14323645298643398No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6200.14277588561348725No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6120.14093361612170033No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGAGTTTTTGTACAATATGTT6110.14070333243522695No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6040.1390913466299134No Hit
CTCCTACTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCG5970.13747936082459983No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5920.136327942392233No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5870.13517652395986618No Hit
CCACTGCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAG5770.1328736870951325No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5750.13241311972218575No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAATACACAG5710.1314919849762923No Hit
TCTGAAAGTAATGATTAACTACATGCTCTGGATAATAGGGTTTGATTCCT5680.1308011339168722No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5670.13057085023039883No Hit
CCTTCTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCGGA5650.1301102828574521No Hit
GGAACATATTGTACAAAAACTCAAATTATGTTTTAGGAAACTTCCTGTAA5620.129419431798032No Hit
ATGCAGTGGAACTCCACCACTTTCCATCAAACGCTTCAAGATCCCAGAGT5590.12872858073861188No Hit
ACTTCCAGACGAGACATTATTTACATACTCTGTGGAAGGCGGGTATCTTA5510.12688631124682495No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5510.12688631124682495No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGAGTTTTTGT5500.1266560275603516No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5480.12619546018740488No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCC5460.12573489281445813No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5460.12573489281445813No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTC5430.12504404175503803No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5430.12504404175503803No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5410.1245834743820913No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5380.1238926233226712No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTATGGA5360.12343205594972446No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5340.12297148857677773No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5290.1218200701444109No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG5210.11997780065262396No Hit
GTACAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGA5190.11951723327967723No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT5180.11928694959320388No Hit
CAGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5140.1183658148473104No Hit
GTATGGATCGGCAGAGGAGACATAAAGGTTCCGCGCATGCGCTGATGGCC5130.11813553116083704No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTCAATC5110.1176749637878903No Hit
TGCGATGGAACTCCCCCACTTTCCATCAAACGCTTCAAGATCCCAGAGTC5060.11652354535552348No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5020.11560241060963No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5000.11514184323668326No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5000.11514184323668326No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTG4990.1149115595502099No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA4990.1149115595502099No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT4970.11445099217726316No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC4970.11445099217726316No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG4950.11399042480431643No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4950.11399042480431643No Hit
CCCCCAACTCCTCCCACTCAGTAAATACACAGTCTTTGAAGTATGCCTCA4870.1121481553125295No Hit
CCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTC4850.11168758793958276No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGAAGATTG4820.11099673688016268No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4730.10892418370190236No Hit
CTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTC4720.108693900015429No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4720.108693900015429No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4640.10685163052364208No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4630.1066213468371687No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4610.10616077946422196No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4580.10546992840480188No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4540.10454879365890841No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4540.10454879365890841No Hit
GTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4540.10454879365890841No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4520.10408822628596168No Hit
CATGTAAACCCTCACAAAACCAAACGATGGGGATACTCCCTTAATTTCAT4520.10408822628596168No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA4460.10270652416712146No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4410.10155510573475464No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTTTGTTTCCCTCAT4400.10132482204828128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)