FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475738
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA24730.5198239367046568No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24260.5099445493107551No Hit
CTCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT19120.4019018871731919No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG15130.3180321941909202No Hit
GTATACATGCATACAAAGGCATTAATGCCGGATAGCCACATTGTGTAAAA14170.29785301993954655No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG14160.29764282020776145No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13550.28482063656886775No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12490.26253946499964265No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGT12370.26001706821822096No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG12320.25896606955929524No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT12020.25266007760574094No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA11530.242360290748269No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11350.23857669557613642No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC11110.23353190201329302No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT10700.2249137130101022No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10440.21944851998368847No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCCGG10350.21755672239762222No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10210.21461392615263025No Hit
GGATCATATTGTACTTAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAA10180.21398332695727482No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10000.21019973178514223No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9920.2085181339308611No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT9470.1990591460005297No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9410.19779794760981886No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA9280.195065351096612No Hit
GTTTCGCTCCCGACCGGCAGCGAGCAAAACAAGCGGCTAGGAGTTCCGCC9250.19443475190125656No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9230.1940143524376863No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT8940.18791856021591719No Hit
ATCATATTGTACTTAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAAAT8850.18602676262985088No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA8740.18371456558021432No Hit
GGGTAAAGGTTGAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA8660.1820329677259332No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8600.18077176933522232No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA8580.18035136987165204No Hit
CATTTGAACCCTAATAAAACTAAACGTTGGGGCTACTCCCTTAACTTTAT8370.17593717550416405No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG8300.17446577738166805No Hit
CCTACACATGAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG8190.1721535803320315No Hit
CTTTTACACAATGTGGCTATCCGGCATTAATGCCTTTGTATGCATGTATA8180.17194338060024636No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7980.1677393859645435No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGC7910.1662679878420475No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7890.16584758837847724No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7870.16542718891490693No Hit
GACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC7830.16458638998776637No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATA7760.16311499186527037No Hit
GTACTTTACCACAGGATCATATTGTACTTAAAATCAAGCAATGTTTTCGA7670.16122319427920412No Hit
GTCTTTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA7520.15807019830242697No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG7480.1572293993752864No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG7460.15680899991171612No Hit
CCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGG7420.15596820098457553No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG7410.1557580012527904No Hit
CAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATA7380.15512740205743497No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7370.15491720232564984No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGT7190.15113360715351729No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7160.15050300795816185No Hit
GTGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTGGGGT7060.14840101064031042No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7040.14798061117674013No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7040.14798061117674013No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6990.14692961251781442No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT6950.14608881359067385No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6940.14587861385888873No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG6930.14566841412710357No Hit
TGGTTATAGCGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCC6900.14503781493174817No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATAC6830.14356641680925217No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6760.14209501868675617No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC6700.1408338202960453No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6630.1393624221735493No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6500.13662982566034246No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6410.13473802807427618No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6390.1343176286107059No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTAT6370.13389722914713562No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGGCGGCCGGAACGG6230.13095443290214362No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6220.13074423317035846No Hit
TTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACAC6220.13074423317035846No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6200.13032383370678818No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6200.13032383370678818No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6170.12969323451143278No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6100.12822183638893678No Hit
ATATTGTACTTAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAAATCGT6090.12801163665715162No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC6060.12738103746179621No Hit
CTTTACCACAGGATCATATTGTACTTAAAATCAAGCAATGTTTTCGAAAA5920.12443824121680422No Hit
CAATAGGACGATTTACAGGAAGTTTTCGAAAACATTGCTTGATTTTAAGT5920.12443824121680422No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5920.12443824121680422No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5900.12401784175323392No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC5860.12317704282609335No Hit
ATGTGGCTATCCGGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGC5800.12191584443538249No Hit
CATGCATACAAAGGCATTAATGCCGGATAGCCACATTGTGTAAAAGGGGC5790.12170564470359735No Hit
CTCAAGGCATACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACC5760.12107504550824193No Hit
CTGTAAGAGGACCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC5760.12107504550824193No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCCGGATAGCCACATTGTGT5730.12044444631288649No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5730.12044444631288649No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5710.12002404684931622No Hit
GGTTAGGATAGAATCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5690.11960364738574593No Hit
GGTAATAGCGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCA5680.11939344765396079No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5660.11897304819039052No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5590.11750165006789452No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC5550.11666085114075396No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5520.11603025194539852No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5470.1149792532864728No Hit
CAGTATGCCTTGAGACTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG5430.11413845435933223No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC5430.11413845435933223No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAAGAGGGA5390.11329765543219167No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC5360.11266705623683623No Hit
GGTTGAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5350.1124568565050511No Hit
ATCTCAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5340.11224665677326597No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5310.11161605757791053No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGTAAGACCTTGGG5250.11035485918719967No Hit
CCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATGTG5190.10909366079648883No Hit
CTATCGTCCCCTTCTGCATCTGCCGTTCCGGCCGCCCACGGGGCGCACCT5190.10909366079648883No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACCGTTGC5170.10867326133291853No Hit
CAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG5160.1084630616011334No Hit
ATATGTAGGTCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA5150.10825286186934827No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5130.10783246240577797No Hit
GCTCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5090.10699166347863741No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5080.10678146374685227No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5060.10636106428328197No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC5060.10636106428328197No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5060.10636106428328197No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCAAGA5050.10615086455149683No Hit
TGGTAATAGCGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCC5020.10552026535614141No Hit
GGGCACACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATC5020.10552026535614141No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4980.10467946642900083No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG4980.10467946642900083No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4950.10404886723364541No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG4930.10362846777007512No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4930.10362846777007512No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAGAGCCCAGTAAAGTTTCCCAC4920.10341826803828998No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA4920.10341826803828998No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTA4860.10215706964757915No Hit
CTTCTGCATCTGCCGTTCCGGCCGCCCACGGGGCGCACCTCTCTTTACGC4850.10194686991579398No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG4840.10173667018400885No Hit
ATGCAGAAGGGGACGATAGAGGCCCAAACGGCCGCGAGACGGGTCGTCCG4800.10089587125686827No Hit
TTGACATACTTTCCAATCAATAGGACGATTTACAGGAAGTTTTCGAAAAC4790.10068567152508315No Hit
GTACAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4790.10068567152508315No Hit
GTACCAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT4770.10026527206151285No Hit
TAATAAAACTAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATGTAA4770.10026527206151285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)