FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475738
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA22820.4796757879336946No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA20520.4313298496231119No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG15950.3352685721973019No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15570.32728098238946646No Hit
GTATACATGCATACAAAGGCATTAATGCCGGATAGCCACATTGTGTAAAA14780.31067520357844025No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13130.27599224783389176No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG12580.2644312625857089No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT11630.24446228806612041No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA11510.24193989128469873No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCCGG11490.24151949182112842No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG10920.22953810710937533No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10910.22932790737759018No Hit
CTCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT10610.2230219154240359No Hit
GGATCATATTGTACTTAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAA10580.22239131622868047No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10040.21104053071228282No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9590.2015815427819514No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG9560.20095094358659596No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGT9370.19695714868267827No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG9350.196536749219108No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA9060.19044095699733887No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT8810.18518596370271032No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8810.18518596370271032No Hit
CTTTTACACAATGTGGCTATCCGGCATTAATGCCTTTGTATGCATGTATA8710.18308396638485888No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8690.1826635669212886No Hit
GGGTAAAGGTTGAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA8660.1820329677259332No Hit
ATCATATTGTACTTAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAAAT8620.1811921687987926No Hit
GTTTCGCTCCCGACCGGCAGCGAGCAAAACAAGCGGCTAGGAGTTCCGCC8620.1811921687987926No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8510.17887997174915604No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8510.17887997174915604No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8460.17782897309023032No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT8420.17698817416308976No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8300.17446577738166805No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC8280.1740453779180978No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8110.17047198247775036No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGC8080.16984138328239493No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA8020.16858018489168408No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7880.16563738864669209No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7670.16122319427920412No Hit
GTACTTTACCACAGGATCATATTGTACTTAAAATCAAGCAATGTTTTCGA7630.16038239535206353No Hit
ATCTCAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG7390.15533760178922013No Hit
CATTTGAACCCTAATAAAACTAAACGTTGGGGCTACTCCCTTAACTTTAT7350.15449680286207954No Hit
GTGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTGGGGT7290.1532356044713687No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT7090.14903160983566585No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7040.14798061117674013No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATA7020.14756021171316985No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6900.14503781493174817No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTAT6900.14503781493174817No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6770.1423052184185413No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG6730.14146441949140073No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG6710.14104402002783042No Hit
GTCTTTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA6680.14041342083247502No Hit
CCTACACATGAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG6670.14020322110068986No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6610.13894202270997902No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6600.13873182297819386No Hit
GGTTAGGATAGAATCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6580.1383114235146236No Hit
ATATTGTACTTAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAAATCGT6560.1378910240510533No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6540.13747062458748302No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6490.1364196259285573No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6490.1364196259285573No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6470.13599922646498702No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6450.13557882700141674No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6420.1349482278060613No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATAC6370.13389722914713562No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6370.13389722914713562No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6370.13389722914713562No Hit
TGGTTATAGCGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCC6280.13200543156106934No Hit
CAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATA6220.13074423317035846No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6170.12969323451143278No Hit
CCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGG6080.12780143692536647No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6060.12738103746179621No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6050.12717083773001106No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6020.12654023853465562No Hit
CTGTAAGAGGACCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC5960.12527904014394478No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5940.12485864068037449No Hit
CTTTACCACAGGATCATATTGTACTTAAAATCAAGCAATGTTTTCGAAAA5910.12422804148501905No Hit
ATGTGGCTATCCGGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGC5880.12359744228966364No Hit
CTATCGTCCCCTTCTGCATCTGCCGTTCCGGCCGCCCACGGGGCGCACCT5880.12359744228966364No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5860.12317704282609335No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5740.12065464604467166No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5720.12023424658110136No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5620.11813224926324993No Hit
GTACCAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT5600.11771184979967966No Hit
CATGCATACAAAGGCATTAATGCCGGATAGCCACATTGTGTAAAAGGGGC5570.11708125060432423No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC5550.11666085114075396No Hit
TTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACAC5530.11624045167718366No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5530.11624045167718366No Hit
ACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAAC5480.11518945301825796No Hit
GTCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT5460.11476905355468767No Hit
GGTTGAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5450.11455885382290253No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5430.11413845435933223No Hit
ATATGTAGGTCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA5420.11392825462754709No Hit
GACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC5410.11371805489576196No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCCGGATAGCCACATTGTGT5400.1135078551639768No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC5400.1135078551639768No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5380.11308745570040653No Hit
CAATAGGACGATTTACAGGAAGTTTTCGAAAACATTGCTTGATTTTAAGT5340.11224665677326597No Hit
GTACAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5330.11203645704148081No Hit
CAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG5310.11161605757791053No Hit
CAGTATGCCTTGAGACTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG5310.11161605757791053No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5290.11119565811434023No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5190.10909366079648883No Hit
GGGCACACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATC5140.10804266213756311No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCAAGA5120.10762226267399283No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC5110.10741206294220768No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5110.10741206294220768No Hit
CTTCTGCATCTGCCGTTCCGGCCGCCCACGGGGCGCACCTCTCTTTACGC5080.10678146374685227No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG5040.1059406648197117No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGT5020.10552026535614141No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAAGAGGGA5010.10531006562435627No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC5000.10509986589257111No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAGAGCCCAGTAAAGTTTCCCAC5000.10509986589257111No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4990.10488966616078597No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4980.10467946642900083No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACCGTTGC4980.10467946642900083No Hit
ATCTTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4950.10404886723364541No Hit
GGTAATAGCGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCA4950.10404886723364541No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4930.10362846777007512No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4860.10215706964757915No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4850.10194686991579398No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATATTGTACTTAAA4840.10173667018400885No Hit
GCTCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4820.10131627072043856No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4800.10089587125686827No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4760.10005507232972771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)