FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540879
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA32740.6053109845270385No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG18280.33796838109817534No Hit
GTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGC17840.3298334747697729No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC17220.3183706522161149No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC17160.31726134680769635No Hit
GAGTACAACAGAGTTGTCTGTTCCGATAAGTTTCGCTCCAGACCGGCTGC17030.3148578517561229No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTAAGAGTCCAA16900.31245435670454946No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16100.2976636179256359No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA15580.28804963771934206No Hit
GCCTCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCC14090.26050188674361546No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG13370.24719022184259326No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13280.24552626372996547No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12650.23387855694157103No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT12650.23387855694157103No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG11880.2196424708668667No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA11880.2196424708668667No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11450.21169244877320068No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11350.20984360642583647No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA11310.20910406948689078No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11210.20725522713952657No Hit
ATTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACA11040.20411219514900747No Hit
CTGTAAGAGGGCCCGCATATTGTTGACATCTATTAATAATGTCCTCCTGT10910.201708700097434No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10810.19985985775006979No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10560.1952377518816593No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGACAAGGGCATTAAACCATAT10490.19394356223850437No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG10370.19172495142166732No Hit
GGACCTTGACCTGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTT10130.18728772978799324No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9960.1841446977974741No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC9770.18063189733748214No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC9740.18007724463327288No Hit
CAGCCATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGT9610.17767374958169943No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA9580.17711909687749017No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG9470.17508537029538954No Hit
GCCTCCGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAA9460.17490048606065314No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8950.1654713900890957No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCGACCTTTAGCCTA8790.162513242333313No Hit
ACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG8760.16195858962910376No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA8750.16177370539436733No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC8740.1615888211596309No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT8660.16010974728173955No Hit
AGAGTACAACAGAGTTGTCTGTTCCGATAAGTTTCGCTCCAGACCGGCTG8630.15955509457753028No Hit
ACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCC8410.15548764141332905No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG8380.15493298870911978No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8380.15493298870911978No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8300.15345391483122842No Hit
CAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTCTAT8210.15178995671860065No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA8060.14901669319755434No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8020.14827715625860868No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA7720.14273062921651608No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGTACAACAGAGT7710.14254574498177966No Hit
GGATACTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCA7660.14162132380809755No Hit
CTTACATAAGAGGACTCTTGGACTCTTAACAATGTCAACGACCGACCTTG7640.1412515553386247No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC7630.1410666711038883No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC7600.14051201839967903No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG7580.1401422499302062No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7470.13810852334810558No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGTCGATGGCC7450.13773875487863274No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7420.13718410217442348No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT7390.13662944947021421No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG7330.13552014406179572No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7290.13478060712285003No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC7240.13385618594916793No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG7180.1327468805407494No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7150.13219222783654017No Hit
CAACAGAGTTGTCTGTTCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCA7090.13108292242812164No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG7080.13089803819338522No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA7060.13052826972391238No Hit
CATTCACTGTAAGAGGGCCCGCATATTGTTGACATCTATTAATAATGTCC7060.13052826972391238No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG7000.12941896431549385No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6810.12590616385550188No Hit
TTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACAC6770.1251666269165562No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT6730.12442708997761052No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6710.12405732150813768No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6660.12313290033445558No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6620.12239336339550991No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6570.1214689422218278No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG6560.12128405798709138No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG6530.12072940528288212No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA6530.12072940528288212No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT6490.11998986834393643No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6470.11962009987446359No Hit
TATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC6440.11906544717025436No Hit
GGTTGGGGCTTGGCTATTGGCCATCGACGCATGCGTGGAACCTTTGTGGC6350.11740148905762657No Hit
GACCTTGACCTGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTT6340.11721660482289015No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6310.11666195211868088No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG6300.11647706788394446No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6220.11499799400605311No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6190.11444334130184385No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6160.11388868859763458No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT6150.11370380436289816No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6140.11351892012816174No Hit
GTCGTTGACATTGTTAAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6070.11222473048500682No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6030.11148519354606114No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT6000.11093054084185187No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5980.11056077237237903No Hit
GTAGAGTCCCAAACGGCCCCGAGATGGGTCGTCCGCGGGATTCAGCGCCG5920.10945146696396052No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5860.108342161555542No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5790.10704797191238705No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG5780.10686308767765063No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5760.10649331920817778No Hit
CCTCAAGGTCGGTCGTTGACATTGTTAAGAGTCCAAGAGTCCTCTTATGT5680.10501424533028644No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5670.10482936109555002No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5650.10445959262607718No Hit
CTCTTACAGTGAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5650.10445959262607718No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5600.10353517145239508No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5580.10316540298292223No Hit
GTCTTACATAAGAGGACTCTTGGACTCTTAACAATGTCAACGACCGACCT5560.10279563451344942No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5550.102610750278713No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCTAGCAGCC5520.10205609757450373No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5510.10187121333976731No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5470.10113167640082163No Hit
TACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACACGC5460.10094679216608521No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGT5440.10057702369661237No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5430.10039213946187595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)