FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540879
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA30250.5592748100776699No Hit
GTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGC20920.3867778190685902No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16790.31042063012244886No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA16770.310050861652976No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTAAGAGTCCAA15700.2902682485361791No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15330.28342753185093156No Hit
GAGTACAACAGAGTTGTCTGTTCCGATAAGTTTCGCTCCAGACCGGCTGC14620.27030075118464575No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC14610.2701158669499093No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG14460.267342603428863No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG13280.24552626372996547No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12760.23591228352367166No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12710.23498786234998956No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC12660.23406344117630745No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG12520.23147506188999759No Hit
GCCTCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCC12000.22186108168370375No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA11880.2196424708668667No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT11100.205221500557426No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11060.2044819636184803No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC10790.19949008928059697No Hit
CTGTAAGAGGGCCCGCATATTGTTGACATCTATTAATAATGTCCTCCTGT10750.1987505523416513No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA10730.19838078387217845No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG10700.19782613116796918No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10440.19301914106482226No Hit
ATTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACA9680.17896793922485435No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC9670.17878305499011793No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9520.17600979146907164No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA9490.17545513876486238No Hit
GGACCTTGACCTGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTT9380.17342141218276177No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9360.17305164371328893No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG9140.16898419054908767No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG9130.16879930631435125No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9060.16750511667119633No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGACAAGGGCATTAAACCATAT9020.16676557973225065No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC8510.15733648376069326No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG8400.15530275717859263No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCGACCTTTAGCCTA8360.15456322023964694No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG8200.15160507248386423No Hit
CAGCCATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGT8110.14994111437123644No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGTCGATGGCC8100.14975623013650002No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8090.1495713459017636No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8060.14901669319755434No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8010.14809227202387226No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG7990.14772250355439942No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7890.1458736612070352No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG7880.1456887769722988No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG7860.14531900850282597No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG7810.14439458732914387No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7790.14402481885967103No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT7780.1438399346249346No Hit
ACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCC7750.14328528192072534No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT7680.1419910922775704No Hit
ACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG7620.14088178686915187No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7620.14088178686915187No Hit
AGAGTACAACAGAGTTGTCTGTTCCGATAAGTTTCGCTCCAGACCGGCTG7610.14069690263441545No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA7600.14051201839967903No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7590.1403271341649426No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG7530.13921782875652408No Hit
GGTTGGGGCTTGGCTATTGGCCATCGACGCATGCGTGGAACCTTTGTGGC7480.138293407582842No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC7470.13810852334810558No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA7420.13718410217442348No Hit
GCCTCCGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAA7380.1364445652354778No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7310.13515037559232287No Hit
GGATACTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCA7140.13200734360180374No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7110.13145269089759448No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC6970.12886431161128462No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6890.12738523773339325No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6850.12664570079444756No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA6810.12590616385550188No Hit
CAACAGAGTTGTCTGTTCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCA6690.12368755303866484No Hit
CAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTCTAT6690.12368755303866484No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6690.12368755303866484No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT6680.12350266880392842No Hit
GTAGAGTCCCAAACGGCCCCGAGATGGGTCGTCCGCGGGATTCAGCGCCG6670.123317784569192No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA6620.12239336339550991No Hit
CATTCACTGTAAGAGGGCCCGCATATTGTTGACATCTATTAATAATGTCC6620.12239336339550991No Hit
CTTACATAAGAGGACTCTTGGACTCTTAACAATGTCAACGACCGACCTTG6590.12183871069130064No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6550.12109917375235496No Hit
TTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACAC6540.12091428951761854No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGTACAACAGAGT6450.11925033140499078No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6440.11906544717025436No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6420.11869567870078152No Hit
GACCTTGACCTGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTT6420.11869567870078152No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6350.11740148905762657No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6330.11703172058815373No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6240.11536776247552595No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6160.11388868859763458No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT6150.11370380436289816No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6100.11277938318921608No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6100.11277938318921608No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT6050.11185496201553398No Hit
GTCTTACATAAGAGGACTCTTGGACTCTTAACAATGTCAACGACCGACCT6010.1111154250765883No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6010.1111154250765883No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5940.10982123543343335No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT5890.10889681425975126No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5880.10871193002501484No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5850.10815727732080557No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5840.10797239308606915No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5840.10797239308606915No Hit
CTCTTACAGTGAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5830.10778750885133273No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5810.10741774038185989No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5770.1066782034429142No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA5770.1066782034429142No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5760.10649331920817778No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5750.10630843497344139No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG5730.10593866650396855No Hit
GTTGTACTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGC5600.10353517145239508No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5580.10316540298292223No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5580.10316540298292223No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC5480.10131656063555805No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5440.10057702369661237No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5440.10057702369661237No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5420.10020725522713952No Hit
AGATAGGGGCATTTGGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACT5420.10020725522713952No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA5410.1000223709924031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)