FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693475
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA40990.591081149284401No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC31520.45452251342874656No Hit
CCTGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC26730.3854500883232993No Hit
AGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA25130.36237787951980965No Hit
GCACCAAACCTCGGCAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT24430.35228378816828293No Hit
CCTCTTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24110.34766934640758496No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA23040.3322398067702513No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT22650.3266159558744007No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20720.29878510400519126No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG20410.29431486354951514No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT20110.2899888243988608No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC18720.26994484300082916No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGACAAAGGCATTAAACCATAT17490.25220808248314647No Hit
TTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACAT16700.24081617938642347No Hit
ATTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA16650.24009517286131438No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC16260.23447132196546377No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA16130.23259670500018026No Hit
GTATACAGGCATATAAAGGCTTTAAGGCAGGATAGCCACATTGTGTAAAA15890.2291358736796568No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15210.2193301849381737No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA15030.21673456144778108No Hit
GTCTCGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14850.2141389379573885No Hit
GTACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT14830.2138505353473449No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT14770.21298532751721405No Hit
GTACTAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT14550.20981289880673418No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA13920.20072821659036016No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA13780.1987093983200548No Hit
TCGGCAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTCT13720.19784419048992394No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT13540.19524856699953136No Hit
CCACCACATTCCACCAAGCTCTGCTAGACCCCAGAGTGAGGGGCCTATAC13450.19395075525433506No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG13190.190201521323768No Hit
GGCTACTCCCTTAATTTCATGGGATATGTAATTGGATGTTGGGGTACTTT13060.18832690435848445No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG12950.18674069000324453No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT12250.17664659865171778No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT12140.17506038429647788No Hit
TTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACAC11870.171166949060889No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG11840.17073434514582356No Hit
AGTCGAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11640.16785031904538736No Hit
TTATATGCCTGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTA11630.16770611774036553No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11550.16655250730019105No Hit
TTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACAT11510.16597570208010382No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCCGACTTCTTTCCTTCTATTCG11400.16438948772486392No Hit
GTATACAGGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11360.16381268250477665No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA11200.1615054616244277No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11080.15977504596416597No Hit
GCTTTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAA10780.15544900681351168No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10720.1545837989833808No Hit
GCCTTAATGCCTTTATATGCCTGTATACAGTCTAAGCAGGCTTTCACTTT10680.15400699376329355No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGTGGTG10670.15386279245827175No Hit
GGAATAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAAG10540.1519881754929882No Hit
GGGAATAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAA10530.15184397418796639No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA10340.149104149392552No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA10050.1449223115469195No Hit
CAAAATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGTGGTG9960.1436244998017232No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT9850.14203828544648328No Hit
GGCTTTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAA9720.14016366848119977No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC9660.1392984606510689No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA9580.1381448502108944No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC9540.1375680449908072No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG9490.1368470384656981No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9430.13598183063556724No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA9350.13482822019539276No Hit
GTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGTGG9240.13324200584015286No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG9050.13050218104473846No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAAGGCAAATATTTGGTAAGGTT8990.1296369732146076No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8980.1294927719095858No Hit
GGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAA8960.12920436929954215No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC8930.1287717653844767No Hit
ATGTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGT8850.12761815494430226No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8830.12732975233425864No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGGCAAGGCATGGGGACGAATCTT8770.12646454450412778No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG8720.12574353797901872No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC8710.12559933667399692No Hit
GTCAAAATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGTGG8680.12516673275893148No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8590.12386892101373519No Hit
TTCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8360.12055229099823353No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8320.11997548577814629No Hit
CCTTTCCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC8300.11968708316810266No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8190.11810086881286275No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8090.11665885576264466No Hit
TTTGCTCAGTGGTTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTAT8060.11622625184757923No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT8040.1159378492375356No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG8030.1157936479325138No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8010.11550524532247017No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG7850.11319802444212121No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT7790.11233281661199035No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG7710.11117920617181584No Hit
CTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCC7700.11103500486679405No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7640.11016979703666319No Hit
GAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGACCCAGCATCCAGGG7430.10714156963120516No Hit
GTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT7390.10656476441111792No Hit
CCTAAAACTCAGACAACTACTGTGGTTTCACATTTCCTGTCTTACTTTTG7360.10613216049605248No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA7310.10541115397094344No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7290.10512275136089981No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7260.10469014744583438No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG7250.10454594614081258No Hit
CTGGCTGGGGCTTGGCAATTGGCCATCGCCGCATGCGTGGAACCTTTGTG7220.10411334222574714No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7220.10411334222574714No Hit
CCACAACATTCCACCAAGCTCTGCTAGACCCCAGAGTGAGGGGCCTATAC7200.10382493961570351No Hit
ATGTTGTGGAGTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAGAGGT7190.10368073831068171No Hit
TACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACACGC7120.10267132917552904No Hit
AAAACTCAGACAACTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA7120.10267132917552904No Hit
ATGTGGTGGAGTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAGAGGT7050.10166192004037637No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC7010.10108511482028913No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6980.10065251090522369No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6970.10050830960020188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)