FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508847
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135142.6558081309313017No Hit
ACTGTGGACTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG14990.2945875675792527No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCCATGAA12660.24879777221836819No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12320.24211599950476273No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTGTCTTCCGAAAGTAAGG12220.24015077223605522No Hit
CTCAAGGACAGTTTGTCTTCCGAAAGTAAGGCAAGAAATATGAAACCACA10440.2051697268530619No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGATGGC10300.20241840867687141No Hit
GTTCATATACTGTTTGCTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA10020.19691577232449045No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA9570.18807224961530677No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9470.18610702234659926No Hit
CTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT9100.17883568145238157No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT8700.1709747723775516No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA8330.16370343148333388No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTGCAAATT8250.1621312496683679No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG8030.15780774967721142No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG8020.1576112269503407No Hit
GTGCTGGTGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGG7910.15544947695476244No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT7910.15544947695476244No Hit
ATCTAGTAGATCCCGGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7820.1536807724129257No Hit
GTATCTAGTAGATCCCGGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGA7790.15309120423231345No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCTGTATCTAGTAGATCCCGGA7750.15230511332483043No Hit
CTTATAAAGAATTTGGAGCTACTGTGGACTTACTCTCGTTTTTGCCTTCT7740.1521085905979597No Hit
TCCCGATACAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAA7680.1509294542367352No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTATTTAGTGCTGTT7490.147195522426191No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7220.14188940880068077No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7150.1405137497125855No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTTTGGGGCATGGACATTGA7040.1383519997170073No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGCCTAAAGATCAGG7000.13756590880952427No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT6940.13638677244829978No Hit
ATGTAAGACCTTGGGCAGGCTCTGATGGGCGTTCACGGTGGTCTCCATGC6650.13068761336904805No Hit
CCCTTATAAAGAATTTGGAGCTACTGTGGACTTACTCTCGTTTTTGCCTT6630.13029456791530655No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGACTTACTCTCGTTT6540.12852586337346983No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6410.1259710679241501No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG6370.1251849770166671No Hit
ATACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCT6220.12223713611360587No Hit
GTCTAGAGGCAAGTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA6140.12066495429863987No Hit
GAATTAATGACTCTAGCTACCTGGGTGGGTAATAATTTGCAAGATCCAGC5990.11771711339557862No Hit
GCCTTAGAGTCTCCTGAGCATTGCACACCTCACCATACAGCACTCAGGCA5970.11732406794183713No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC5950.11693102248809563No Hit
TGTTAATACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATA5550.10907011341326567No Hit
GTCCACAGTAGCTCCAAATTCTTTATAAGGGTCAATGTCCATGCCCCAAA5510.10828402250578269No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAATGCTTCCGGA5450.1071048861445582No Hit
GGGTAAAGGTTCATATACTGTTTGCTTAGAAAGGCCTTGTAAGTTGGCGA5430.10671184069081668No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAATCT5430.10671184069081668No Hit
GGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT5270.1035674770608847No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG5150.10120920433843571No Hit
CCATTTACACAATGTGGTTACCCTGCCTTAATGCCTTTGTATGCATGTAT5130.10081615888469422No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGAA5110.10042311343095273No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA5100.10022659070408196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)