FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455350
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27160.5964642582628747No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGTATGTTCAGG10320.22663884923685076No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9510.20885033490721425No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8990.19743054793016362No Hit
ATCCTAACCTCACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8870.19479521247392115No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7820.1717360272317997No Hit
CTCTTACAGTAAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7620.16734380147139563No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT7540.16558691116723398No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7020.15416712419018339No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7010.15394751290216316No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6900.1515317887339409No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6180.1357197759964862No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA6170.13550016470846601No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6170.13550016470846601No Hit
GGAACATATTGTGCAAAAATTGAAACACTGTTTTCGGAAACTTCCTGTAA5850.12847260349181946No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGTA5650.12408037773141539No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT5570.12232348742725375No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATTGGAGGAC5510.12100581969913253No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA5420.1190293181069507No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCTTCTTTTCCTCAC5240.115076314922587No Hit
GCAATAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5140.11288020204238498No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG5100.11200175689030417No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5100.11200175689030417No Hit
GTTCTATCCTAACCTCACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5050.11090370045020313No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4960.10892719885802131No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4910.10782914241792027No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTG4900.10760953112990007No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4900.10760953112990007No Hit
CATGTATACAAGCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4880.10717030855385966No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACAGACT4790.10519380696167784No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTGAGGTT4750.104315361809597No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT4750.104315361809597No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT4740.1040957505215768No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATGTCTTCTTTC4660.10233886021741517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)