FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449811
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38080.8465777848918767No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA14100.3134649886285573No Hit
CAATTACATATCCCATAAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG10940.24321326068059695No Hit
ACGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCC10730.2385446331903844No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10210.22698422226223902No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAAAGTCCAA10200.22676190666746698No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTGACCTT10180.22631727547792296No Hit
CTTTTATATAAGATCCCCGCCTTCCACAGAACGTGTAAATAATGTCGTGT9730.21631307371318176No Hit
CTCCAAAAGACACCAAATAATTAAGAACAGTTTCTCTTCCAAAAGTAAGA9570.21275602419682932No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9410.20919897468047693No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9190.20430803159549232No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGTACAATATGTT9100.20230719124254407No Hit
CCAAAAGACACCAAATAATTAAGAACAGTTTCTCTTCCAAAAGTAAGACA9040.20097329767391195No Hit
GGAACATATTGTACAAAAACTCAAAATGTGTTTTAGGAAACTTCCTGTAA8840.19652698577847139No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA8450.18785667758236238No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCATAAAAATCAGA8330.18518889044509806No Hit
CCCTTACAGTAAATGAAACCAGGAGATTAAAATTAATTATGCCTGCTAGG8180.18185415652351766No Hit
AGTCTGTACAACATCTTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT8160.18140952533397361No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7820.17385079511172472No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7790.17318384832740863No Hit
GTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCT7590.1687375364319681No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7540.16762595845810796No Hit
ATTAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7370.1638465933469835No Hit
CATTTGAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTTAT7180.159622597046315No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7050.15673249431427866No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATAAA6820.15161923563452204No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACC6810.15139692003975003No Hit
CGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6700.14895144849725775No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6490.14428282100704518No Hit
GTACAACATCTTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6440.14317124303318507No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACACGACATTATT6250.13894724673251654No Hit
GAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCATAAAAATCAGACA6170.13716872197434032No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTCAAATGTATAC5980.13294472567367183No Hit
GTATAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC5860.1302769385364075No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTG5800.12894304496777537No Hit
ATAATTAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACC5620.12494136426187887No Hit
GCCTTAGAGTCTCCTGAACATTGTTCATATCACCATACGGCAATAAGGCA5570.12382978628801874No Hit
GGGTATACATTTGAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA5390.11982810558212227No Hit
GGATATCCACATTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAAT5380.11960578998735025No Hit
ATGTCAACGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCAC5240.11649337166054188No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGT5240.11649337166054188No Hit
GTCGTTGACAGTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5160.11471484690236566No Hit
GCTTTAATCCTGACTGGCAGACTCCTTCTTTTCCTGATATTCATTTGCAG5140.1142702157128216No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTGTTTACTGAGT5130.11404790011804958No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAGATACCCGTG5030.11182474417032932No Hit
CCACAATAGTTGTCTGATTTTTATGCCCATATTAACGTTGACATAGCCGA5010.11138011298078526No Hit
CTCTTTTATATAAGATCCCCGCCTTCCACAGAACGTGTAAATAATGTCGT5000.11115779738601324No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAG5000.11115779738601324No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGAATA4970.11049085060169715No Hit
TATCCACATTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG4910.10915695703306501No Hit
TATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTCC4900.10893464143829298No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGC4890.10871232584352093No Hit
GTTGTGAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4740.10537759192194056No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4690.10426601394808042No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAGGAGATTA4650.10337675156899231No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4600.10226517359513218No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT4600.10226517359513218No Hit
GTCTTTGGGTATACATTTGAACCCTCACAAAACAAAAAGATGGGGATATT4600.10226517359513218No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4590.10204285800036014No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4580.10182054240558812No Hit
CAGTAAATGAAACCAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTAT4570.1015982268108161No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4520.10048664883695597No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4510.10026433324218392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)