FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429483
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN64621.5045997164032103No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10450.24331580062540312No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8710.20280197353562307No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7310.17020464139442074No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA7130.166013555833409No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7110.16554787965996326No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6850.15949408940516854No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6840.15926125131844568No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6440.14994772784953073No Hit
CTCCTAGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCTGA6300.1466879946354105No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGCAAGTCCAA6140.1429625852478445No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG6060.1410998805540615No Hit
GTCTAGGAGATCGCGAATAGAAGGAAAAAAGTCAGCAGGCAAAAAAGAGA6000.13970285203372426No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5870.13667595690632692No Hit
GGAACATATTGTACAAAAAATCAAACTGTGTTTTCGGAAACTTCCTGTAA5720.13318338560548382No Hit
ATTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAA5580.12992365239136355No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5280.12293850978967735No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4930.11478917675437678No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4870.11339214823403952No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4820.11222795780042516No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4660.1085025484128592No Hit
GTTTACAGGAAGTTTCCGAAAACACAGTTTGATTTTTTGTACAATATGTT4540.10570849137218469No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4450.10361294859167884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)