FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429483
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN108912.535839602498818No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10580.24634269575280046No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA7310.17020464139442074No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG7250.1688076128740835No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6870.1599597655786143No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT6690.15576868001760258No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGCAAGTCCAA6690.15576868001760258No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6510.15157759445659083No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6290.1464551565486876No Hit
CTCCTAGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCTGA6250.14552380420179611No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6020.14016852820717002No Hit
GTCTAGGAGATCGCGAATAGAAGGAAAAAAGTCAGCAGGCAAAAAAGAGA6020.14016852820717002No Hit
ATTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAA5510.12829378578430345No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5490.12782810961085772No Hit
GGAACATATTGTACAAAAAATCAAACTGTGTTTTCGGAAACTTCCTGTAA5240.12200715744278587No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5120.11921310040211136No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5080.11828174805521988No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4870.11339214823403952No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4700.10943390075975068No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4690.10920106267302779No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4590.10687268180579906No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4550.10594132945890758No Hit
GTTTACAGGAAGTTTCCGAAAACACAGTTTGATTTTTTGTACAATATGTT4360.10151740581117297No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4340.10105172963772721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)