FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188205
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11700.6216625488164502No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGTAGTAATGATTAACTAGA5750.3055179192901358No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4120.21891023086527986No Hit
CTCTATAACCGTGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4080.21678488881804414No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT3970.21094019818814588No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3910.20775218511729232No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGTGTTCACGGTGGTCTCCATGC3620.1923434552748333No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGAACACCGTG3590.1907494487394065No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACGTAGCGCC3520.18703010015674398No Hit
CCCTTATCCAATGGCAAATATTTGGTACCCTTCGGATAAAACCTAGCAGG3190.1694960282670492No Hit
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC2790.14824260779469195No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2660.14133524614117585No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC2520.1338965489758508No Hit
GCGTTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT2460.13070853590499723No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT2460.13070853590499723No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTACCCTTCGGATAAAAC2380.12645785181052577No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT2370.12592651629871685No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC2350.12486384527509896No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT2330.1238011742514811No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT2320.12326983873967216No Hit
GTACAACATCTTGAGTCCCTTTACACCGCTGTTACCAATTTTCTTTTGTC2300.1222071677160543No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC2240.11901915464520071No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT2210.11742514810977392No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTACCCTT2200.116893812597965No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGGA2190.11636247708615606No Hit
CTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAC2160.11476847055072925No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGT2150.11423713503892031No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT2140.11370579952711139No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT2080.11051778645625779No Hit
CTTACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT2010.10679843787359529No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT2000.10626710236178635No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGTGTTC1980.10520443133816848No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGGCTTTATTC1980.10520443133816848No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT1940.10307908929093276No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA1930.10254775377912383No Hit
CTCTAAGGCATCCCGAAATAGAGCTGAGGCGGTATCTAGAAGATCCCGTA1920.1020164182673149No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTG1920.1020164182673149No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA1920.1020164182673149No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1900.10095374724369705No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA1890.1004224117318881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)