FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273144
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA9560.3499985355709809No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT8000.2928858038250886No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA7270.26615997422604926No Hit
GCGTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAA7060.2584717218756407No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6840.25041736227045075No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6570.240532466391354No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGATTC6570.240532466391354No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6050.22149488914272325No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT5960.218199923849691No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5420.1984301320914975No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5180.18964355797674487No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5000.18305362739068037No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTACGATGTGTT4990.182687520135899No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT4970.1819553056263363No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAATCAATTG4840.1771959113141786No Hit
CATTTAAACCCTGACAAAACAAAGAGATGGGGTTACTCTCTACATTTTAT4720.17280262425680226No Hit
GCACCTGGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4680.17133819523767682No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4650.17023987347333275No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG4620.16914155170898867No Hit
GTGTTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGATTCTG4440.16255162112292418No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC4170.15266672524382743No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4060.14863954544123245No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4000.1464429019125443No Hit
GGCTTTCACTTTCTCGCCAACCTATAAGGCCTTTCTGTGTAAACAATACC3950.1446123656386375No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC3920.1435140438742934No Hit
AGTCTAGATTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3860.14131740034560525No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC3780.13838854230735437No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3760.13765632779779163No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT3720.13619189877866622No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3720.13619189877866622No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA3670.13436136250475939No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA3560.13033418270216443No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT3540.1296019681926017No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATGCGTTGACATACT3530.12923586093782036No Hit
CCTATAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCC3500.12813753917347626No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTT3420.12520868113522537No Hit
GGGAGATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT3240.11861875054916089No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT3170.11605599976569135No Hit
GCCTAGCAGCCATGGAAAGGATGTATATTTGCGGGAGAGGACAACAGAGT3140.11495767800134726No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3100.11349324898222184No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT3100.11349324898222184No Hit
GGGGAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT3080.1127610344726591No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT3080.1127610344726591No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC3050.11166271270831503No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA3050.11166271270831503No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3040.11129660545353368No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA3040.11129660545353368No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAGGTTGGCGA3030.1109304981987523No Hit
CCTTTAAGAGACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTT3020.11056439094397094No Hit
GGAAACACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCA2980.1090999619248455No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC2960.10836774741528278No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCATTAAC2920.10690331839615734No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2850.10434056761268781No Hit
GAGTAACCCCATCTCTTTGTTTTGTCAGGGTTTAAATGTATACCCAAAGA2850.10434056761268781No Hit
GTCCCAGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCCA2840.10397446035790646No Hit
ATCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG2820.10324224584834372No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA2800.102510031338781No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA2800.102510031338781No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC2800.102510031338781No Hit
CTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC2780.1017778168292183No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2770.10141170957443692No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT2770.10141170957443692No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2760.10104560231965556No Hit
CCCCTATCCTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC2750.10067949506487421No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT2750.10067949506487421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)