FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317646
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA12760.4017050427205128No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10390.32709368290486895No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9280.29214912197855475No Hit
CTCTATGGAAGGCGGGTATATTATATAAAAGAGAAACAACACGTAGCGCC8820.2776675922253074No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8350.2628712466078591No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCATTTCCTAAT7890.24838971685461173No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7340.23107484432355516No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA6870.2162784987061068No Hit
GTTTCCTAAAACAGTCTTTGATTTTCTGTTTGATGTGCTCTTGTGGCAAT5670.17850059500198334No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCCCCTAGTACAAAGATC5670.17850059500198334No Hit
GAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCC5470.17220427771796276No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5390.16968575080435452No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5360.16874130321175143No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGGAT5090.16024127487832368No Hit
CTCAAGCTCAGGGCATACTACAAACCTTGCCAGCAAATCCGCCTCCTGCA4920.1548894051869062No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4820.1517412465448959No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4740.14922271963128766No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4680.14733382444608148No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT4660.14670419271767943No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTTAT4650.1463893768534784No Hit
GGTTATAGCATTGGATGTTATGGGTCATTGCCACAAGAGCACATCAAACA4310.1356856374706434No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA4270.1344263740138393No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4210.13253747882863315No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA4140.13033376777922592No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC4040.12718560913721563No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC4000.12592634568041153No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA3960.12466708222360742No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC3920.1234078187668033No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAACGCCGCA3910.12309300290260228No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG3890.12246337117420021No Hit
TTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG3840.12088929185319507No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCATTTCCTAATATACATTTACA3790.11931521253218992No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3790.11931521253218992No Hit
CTTTAGGACATGGACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGA3790.11931521253218992No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC3760.11837076493958684No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT3730.11742631734698375No Hit
ATATAAAAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCGCCATATT3730.11742631734698375No Hit
GGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCGTTTTTCATTGAC3710.1167966856185817No Hit
GGGTAAAGGTTCAGATATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA3700.11648186975438066No Hit
CCGATAAGGTTTGCTCCAGACCTGCTGCGAGCAAAGCAAGCGGCTAGGAG3640.11459297456917449No Hit
GAGCTTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCC3560.11207444765556626No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA3560.11207444765556626No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCATTTC3510.11050036833456112No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCGTTTTTCA3510.11050036833456112No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3480.10955592074195802No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG3410.10735220969255084No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3410.10735220969255084No Hit
CTCACAGTCAATGAAAAACGAAGACTACAATTAATTATGCCTGCTAGGTT3400.10703739382834979No Hit
GCTATAACCCATAAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG3390.10672257796414876No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC3380.10640776209994773No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3320.10451886691474158No Hit
GTGTAAATGTATATTAGGAAATGAGGGTGTTTTCCAATGAGGGTTAAAGA3310.10420405105054055No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG3260.1026299717295354No Hit
TTAGAAAACTTCCTGTTAACAGGCCAATTGATTGGAAAGTCTGTCAGCGT3240.10200034000113334No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC3220.10137070827273129No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT3200.1007410765443292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)