FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317646
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA13270.41776065179476524No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10420.328038130497472No Hit
CTCTATGGAAGGCGGGTATATTATATAAAAGAGAAACAACACGTAGCGCC9220.29026022679334856No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCATTTCCTAAT8590.27042682734868373No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8500.2675934845708745No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8220.2587786403732457No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7640.24051932024958603No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA7640.24051932024958603No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC6220.19581546753303994No Hit
GTTTCCTAAAACAGTCTTTGATTTTCTGTTTGATGTGCTCTTGTGGCAAT5950.18731543919961213No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5900.18574135987860702No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCCCCTAGTACAAAGATC5260.16559314456974114No Hit
GAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCC5150.16213017006352984No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGGAT5130.16150053833512779No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTTAT5130.16150053833512779No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT5070.15961164314992163No Hit
CTTTAGGACATGGACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGA4990.1570931162363134No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA4860.1530005100017No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4800.15111161481649382No Hit
GGTTATAGCATTGGATGTTATGGGTCATTGCCACAAGAGCACATCAAACA4440.1397782437052568No Hit
CTCAAGCTCAGGGCATACTACAAACCTTGCCAGCAAATCCGCCTCCTGCA4380.13788934852005064No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC4280.13474118987804035No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4240.1334819264212362No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA4180.13159303123603006No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4010.12624116154461257No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3950.12435226635940638No Hit
GGGTAAAGGTTCAGATATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA3920.1234078187668033No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCGTTTTTCA3920.1234078187668033No Hit
CTTTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3870.12183373944579816No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA3820.12025966012479301No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA3810.11994484426059199No Hit
TTAGAAAACTTCCTGTTAACAGGCCAATTGATTGGAAAGTCTGTCAGCGT3790.11931521253218992No Hit
GGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCGTTTTTCATTGAC3770.11868558080378787No Hit
GAGCTTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCC3690.11616705389017963No Hit
GCATTGGATGTTATGGGTCATTGCCACAAGAGCACATCAAACAGAAAATC3620.11396334284077243No Hit
ATATAAAAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCGCCATATT3600.11333371111237037No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3560.11207444765556626No Hit
GCTATAACCCATAAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG3540.1114448159271642No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG3520.11081518419876214No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCATTTCCTAATATACATTTACA3500.11018555247036008No Hit
TTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG3460.10892628901355599No Hit
GTGTAAATGTATATTAGGAAATGAGGGTGTTTTCCAATGAGGGTTAAAGA3450.10861147314935493No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG3440.1082966572851539No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA3400.10703739382834979No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA3340.10514849864314361No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3330.10483368277894259No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3310.10420405105054055No Hit
CCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT3300.1038892351863395No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG3280.10325960345793746No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC3230.10168552413693231No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG3220.10137070827273129No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3190.1004262606801282No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3180.10011144481592717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)