FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367993
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA24930.6774585386135062No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13590.3693005029986983No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC10580.2875054688540271No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9760.2652224362963426No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCGAGTACAAAGACC9750.2649506919968586No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT9370.2546244086164683No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8660.23533056335310726No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8140.22119985977994147No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT8000.21739543958716606No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCAT7700.20924311060264733No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7590.2062539233083238No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7340.19946031582122487No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGCCCACT7280.19782985002432113No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7140.19402542983154572No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6700.18206868065425158No Hit
CTCACTGTTAGTATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCT6470.17581856176612057No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5960.1619596024924387No Hit
CTCTATGACTATATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5700.1548942507058558No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5540.15054634191411248No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA5520.15000285331514457No Hit
GCTTAGATTGAATACAGGCATACAAGGGCATTAAGGCAGGATAACCACAT5330.14483971162494938No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5170.14049180283320606No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5070.1377743598383665No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4910.13342645104662318No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT4870.13233947384868733No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC4820.13098075235126755No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4790.13016551945281568No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4770.12962203085384777No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4750.12907854225487986No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCCTGTATT4730.12853505365591195No Hit
ATTAATTGCAGTCTTCGTTTCTCATTTACTGTGAGTGGGCCTACAAATTG4660.12663284355952423No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4640.12608935496055632No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT4610.12527412206210445No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4530.12310016766623277No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCGGTACG4520.12282842336674882No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTTAC4490.12201319046829695No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC4440.12065446897087716No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4440.12065446897087716No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT4430.1203827246713932No Hit
CAGTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT4340.11793702597603758No Hit
ATACAAACCTTACCAGCAAATCCGCCTCCTGCGTCTACCAATCGCCAGTC4340.11793702597603758No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4240.11521958298119801No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC4210.11440435008274613No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA4200.11413260578326218No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG4200.11413260578326218No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4180.11358911718429426No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4170.11331737288481031No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4140.11250213998635844No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4110.11168690708790656No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTTACTGTGAGTGGGCCTAC4030.1095129526920349No Hit
GTTAGTATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTC3980.10815423119461512No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3980.10815423119461512No Hit
CCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG3960.1076107425956472No Hit
ATGTCATTGGATGTCATGGGTCATTGCCACAAGATCACATCATACAGAAA3940.10706725399667928No Hit
GACTATATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTT3940.10706725399667928No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT3890.1057085324992595No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT3890.1057085324992595No Hit
GTTTACTAGTGCCCTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3750.1019041123064841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)