FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362613
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTAAACCCTCATAAAACAAAAAGATGGGGCTATTCCCTTAACTTCAT10640.2934257734830246No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9990.27550032679468195No Hit
GGATAATAAGGTTTAATTCCTTTATCTAAGGGCAAATATTTGGTAACCTT9310.2567475517976465No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9230.2545413429744659No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGT8660.23882210510930385No Hit
ACTCACAACTGTGCCAGCATCTCCTCCTCCTGCCTCCACCAATCGGCAGG8560.23606434408032806No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAA8120.22393019555283455No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAA7650.21096871871664832No Hit
CATACAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGAGCAGCA7440.20517742055579918No Hit
ATCCTAAGGTTACCAAATATTTGCCCTTAGATAAAGGAATTAAACCTTAT6590.1817364518095049No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6550.1806333473979146No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA6260.17263584041388477No Hit
GGGTAAAGGTTCAGATACTGTTTGTTTAGAAAAGCCTTGTAAGTTGGCGA6260.17263584041388477No Hit
TCCAAAGAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA6220.17153273600229446No Hit
GTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCTGG6200.17098118379649932No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC6100.1682234227675235No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCTCCATG5930.16353522901826464No Hit
CCTTAGGATAAAACCTAGCAGGCATGATCAATTTTAGTCTCCTGTTTTCA5770.15912281137190337No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA5760.1588470352690058No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5690.15691660254872275No Hit
GGATATTGTTGATAGGTGTAAACAATTTGTGGGACCCCTCACAGTAAATG5510.1519526326965663No Hit
CTGTACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTG5430.14974642387338566No Hit
GTTCAGAACACTGCCTCTTCCATATCGTCAATCTTATCGAAGACTGGGGA5400.14891909556469296No Hit
GGTGTAAACAATTTGTGGGACCCCTCACAGTAAATGAAAACAGGAGACTA5220.14395512571253652No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACACTGTACACCTCACCATACGG5090.14037003637486797No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACTTTACCCCAG5070.13981848416907283No Hit
GGATAAAACCTAGCAGGCATGATCAATTTTAGTCTCCTGTTTTCATTTAC5050.13926693196327763No Hit
GGAACATATTGTGTTGAAAATCAAACAATGTTTTAGAAAACTTCCTGTAA5030.1387153797574825No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATT5010.13816382755168732No Hit
GTTCAGATACTGTTTGTTTAGAAAAGCCTTGTAAGTTGGCGAGAAAGTAA4960.13678494703719943No Hit
GTATACATCTAAACCCTCATAAAACAAAAAGATGGGGCTATTCCCTTAAC4870.13430296211112122No Hit
GTTGATAGGTGTAAACAATTTGTGGGACCCCTCACAGTAAATGAAAACAG4850.13375140990532605No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCT4850.13375140990532605No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATCTAA4790.1320967532879406No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCGGGC4720.13016632056765753No Hit
GTTCTGAACAGGGTTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4680.1290632161560672No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4630.1276843356415793No Hit
GCCTTAGAGTCTCCGGAACACTGTACACCTCACCATACGGCACTCAGGCA4500.12409924630391077No Hit
CTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACT4490.12382347020101321No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTACGGGACAGGACGACAGAAT4430.12216881358362772No Hit
AGATAGGGGCATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACT4340.11968682865754951No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4310.11885950034885677No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4180.11527441101118824No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA4170.11499863490829067No Hit
CCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAACTCCACCACGTT4110.11334397829090517No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4100.11306820218800759No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGTCTGGA4100.11306820218800759No Hit
CCTTATGAGTCCACGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4030.11113776946772454No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTATTAGACGAAGAGGC4020.11086199336482697No Hit
TCCTCAAGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG4010.11058621726192938No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG3990.11003466505613423No Hit
CAATAGGCCTATTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAAC3990.11003466505613423No Hit
GCTTTATTCTTCTACGGTACCGGGCTTTAATCCTGAATGGCAAACTCCTT3980.10975888895323664No Hit
GTGTGAAAGGAGCAGCAAATCCCAAAAGACCTACAATTCGTTGACACACT3930.10838000843874875No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACGTTTCC3890.10727690402715843No Hit
ATATGTTCCTGGGGTAAAGTGCCCCAACTTCCAATTACATATCCCATGAA3860.1064495757184657No Hit
GTTTTATCCTAAGGTTACCAAATATTTGCCCTTAGATAAAGGAATTAAAC3810.1050706952039778No Hit
GGATAACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCTACAAT3760.1036918146894899No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3750.1034160385865923No Hit
GGATAAGATAGGGGCATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCC3730.10286448638079715No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3720.10258871027789959No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3720.10258871027789959No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3710.10231293417500201No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT3700.10203715807210441No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3680.10148560586630925No Hit
GGTTCAGATACTGTTTGTTTAGAAAAGCCTTGTAAGTTGGCGAGAAAGTA3670.1012098297634117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)