FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592578
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA42470.7166988987103807No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37610.6346843791028355No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC28280.4772367519550169No Hit
ATCCTAATGTTAGTATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGG26390.44534221655208256No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGG25550.4311668674841118No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC24960.42121037230541797No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT21370.3606276304554No Hit
GAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAATA17520.2956572805605338No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAGTTTGTAGGCCCACT16910.28536327707069786No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13570.22899938910995685No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT13470.22731184755424602No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12940.21836787730897872No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT12940.21836787730897872No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT12890.21752410653112336No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA12590.2124614818639909No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT11890.20064869097401522No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG11830.19963616604058873No Hit
CATTAGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT11530.1945735413734563No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC11060.18664209606161553No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA10980.18529206281704685No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC10920.1842795378836204No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCTTGTATT10620.17921691321648797No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10540.17786687997191933No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG10410.17567307594949524No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTT10100.17044169712679175No Hit
GCTGTAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAAC10070.16993543466007852No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT9960.1680791389487966No Hit
ATCCAAAGGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT9760.164704055837375No Hit
ACAAAGAGATGGGGTTACTCGCTAAATTTTATGGGTTATGTCATTGGAAG9720.16402903921509068No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9710.16386028505951958No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT9700.1636915309039485No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9460.15964143117024257No Hit
GTTTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA9440.15930392285910042No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9190.15508506896982338No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAATCAATTG9180.1549163148142523No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA9150.15441005234753905No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG8800.1485036569025512No Hit
ATTAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAAAAAAG8770.14799739443583798No Hit
AGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAA8600.14512857379112962No Hit
TCTCCTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAAT8550.1442848030132742No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8530.14394729470213202No Hit
CCTACAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA8450.14259726145756338No Hit
AATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTTGTCT8220.13871591587942853No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA8190.1382096534127153No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTACGATGTGTT7980.1346658161457226No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7880.13297827459001177No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT7850.13247201212329854No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT7780.13129073303430097No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC7670.1294344373230191No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT7620.12859066654516368No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG7280.12285302525574693No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7080.1194779421443253No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA7050.11897167967761205No Hit
GGCCCATGGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG6970.11762164643304343No Hit
GAAACGATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGATA6940.11711538396633016No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTACCATTGG6880.11610285903290368No Hit
CTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT6780.11441531747719287No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6760.11407780916605072No Hit
TTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATG6700.11306528423262421No Hit
CACCAAGACATTATTAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT6570.11087148021020019No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT6560.1107027260546291No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGAATTGTGGGTCT6490.10952144696563153No Hit
GAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAATA6490.10952144696563153No Hit
GAATACAAGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6470.10918393865448936No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC6470.10918393865448936No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC6440.10867767618777611No Hit
CTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC6410.10817141372106288No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6370.10749639709877856No Hit
GACCCATAACATCCAATGACATAACCCATAAAATTTAGCGAGTAACCCCA6360.10732764294320747No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACC6340.10699013463206532No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6270.10580885554306776No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCATTAAC6220.10496508476521235No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCGCTAAATTTTAT6180.10429006814292802No Hit
CTCTATAACCGTTTGTGTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6060.10226501827607505No Hit
TTATTAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAAAAA5980.1009149850315064No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5980.1009149850315064No Hit
CTATAACCGTTTGTGTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG5970.10074623087593532No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5940.10023996840922207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)