FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191488
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA45842.393883689839572No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTCCTAAT21721.1342747326203209No Hit
CCTGCCTTGTCGTCTAATAACAGTAGTCTCCGGAAGTGTTGATAAGATAG20541.0726520721925135No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT16240.8480949197860962No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14410.7525275735294118No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13690.7149273061497327No Hit
ACAATACAGGTCCTCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT11330.5916819852941176No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT11090.5791485628342247No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10510.5488594585561497No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCATTTCCTAATATACATTTACA9640.5034258021390374No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC9430.49245905748663105No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8430.44023646390374327No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTC8370.43710310828877No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7780.40629177807486627No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7610.3974139371657754No Hit
CTTTAACCCTCATTGGAAAACACCTTCATTTCCTAATATACATTTACACC7190.3754804478609626No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6860.35824699197860965No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6710.35041360294117646No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT6680.34884692513368987No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6560.34258021390374327No Hit
GATCCAGCATCTAGGGACCTAGTAGTGAGTTATGTCAACACTAATATGGG6500.3394468582887701No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTCCTAATATACA6460.33735795454545453No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6310.3295245655080214No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA6050.3159466911764706No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5940.3102022058823529No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTCCT5790.3023688168449198No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5720.29871323529411764No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5370.28043532754010697No Hit
CCTGTATTGTCGTCTAATAACAGTAGTCTCCGGAAGTGTTGATAAGATAG5210.27207971256684493No Hit
ACTACACACCGTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGT5140.2684241310160428No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCTCCGTG5040.263201871657754No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTTAT4960.259024064171123No Hit
GCATAGCCTGAGGATGAGTTTGTCTCAAAGGTGGAGACAGCGGGGTAGGC4850.25327957887700536No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA4840.25275735294117646No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4740.2475350935828877No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACCACCGTG4560.2381350267379679No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4460.23291276737967917No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA4410.23030163770053477No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4400.2297794117647059No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA4380.22873495989304812No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4160.21724598930481284No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA4120.21515708556149732No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG3990.20836814839572193No Hit
GTCAATGAGAAACGAAGACTACAATTAATTATGCCTGCTAGGTTTTATCC3930.20523479278074866No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC3880.20262366310160426No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG3860.20157921122994651No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3700.19322359625668448No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3660.19113469251336898No Hit
TCCTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTG3650.1906124665775401No Hit
CTCACAGTCAATGAGAAACGAAGACTACAATTAATTATGCCTGCTAGGTT3600.1880013368983957No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3600.1880013368983957No Hit
ACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTCCTAATAT3520.1838235294117647No Hit
TTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCAT3340.17442346256684493No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT3340.17442346256684493No Hit
CTTTATGCCCATATTAGTGTTGACATAACTCACTACTAGGTCCCTAGATG3260.17024565508021391No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3230.16867897727272727No Hit
GGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTT3210.16763452540106952No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3200.16711229946524064No Hit
GAATATGGTGACCCACAAAATGAGGCGCTACGTGTTGTTTCTCTCTTATA3200.16711229946524064No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT3130.1634567179144385No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA3130.1634567179144385No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC3120.16293449197860962No Hit
CATTGGAAAACACCTTCATTTCCTAATATACATTTACACCAAGACATTAT3110.16241226604278075No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG3050.1592789104278075No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCGTTTCTCA3020.15771223262032086No Hit
GTTAACAGGTAGTTTTCTAAAACAGTCTTTGATTTTCTGTATGATGTGAT3010.15719000668449198No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2990.1561455548128342No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG2990.1561455548128342No Hit
CAATACAGGTCCTCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGATC2950.1540566510695187No Hit
GTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATG2900.15144552139037432No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA2900.15144552139037432No Hit
ATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC2840.14831216577540107No Hit
CCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACT2830.1477899398395722No Hit
CCTTCATTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGTGA2730.14256768048128343No Hit
GTGTAAATGTATATTAGGAAATGAAGGTGTTTTCCAATGAGGGTTAAAGA2710.14152322860962568No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTACGGGAGAGAACAACAGAGT2700.14100100267379678No Hit
ATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAA2670.13943432486631016No Hit
GTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCTCCGTGCG2670.13943432486631016No Hit
GGAAAACACCTTCATTTCCTAATATACATTTACACCAAGACATTATCAAA2660.1389120989304813No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC2560.1336898395721925No Hit
AGAATATGGTGACCCACAAAATGAGGCGCTACGTGTTGTTTCTCTCTTAT2550.13316761363636362No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA2540.13264538770053477No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT2460.12846758021390373No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTAT2460.12846758021390373No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG2450.12794535427807485No Hit
CTACAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTT2410.12585645053475936No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC2410.12585645053475936No Hit
GTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATACC2370.12376754679144385No Hit
AACTAGATGTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAAT2370.12376754679144385No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT2360.12324532085561497No Hit
TTGGAAAACACCTTCATTTCCTAATATACATTTACACCAAGACATTATCA2360.12324532085561497No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2350.12272309491978609No Hit
ATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTT2340.12220086898395721No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG2290.11958973930481284No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG2280.11906751336898395No Hit
AGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAGATCCC2280.11906751336898395No Hit
TTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCT2280.11906751336898395No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2250.11750083556149732No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCT2250.11750083556149732No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA2240.11697860962566844No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT2240.11697860962566844No Hit
CCTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGT2220.1159341577540107No Hit
ACGTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA2210.11541193181818182No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC2210.11541193181818182No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA2200.11488970588235295No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG2190.11436747994652406No Hit
ATACTACACACCGTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCA2180.11384525401069519No Hit
GTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGTATGATGTGAT2180.11384525401069519No Hit
ATGCTATAGCCCATAAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTT2180.11384525401069519No Hit
GCTATAGCCCATAAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG2180.11384525401069519No Hit
ATACTGCACTCAGGCAAGCAATTATTTGCTGGGGGGAACTAATGACTCTA2180.11384525401069519No Hit
GCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAAC2170.11332302807486631No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC2160.11280080213903743No Hit
ATCCAATGCTATAGCCCATAAAATTTAAAGAGTAACCCCATCTTTTAGTT2150.11227857620320855No Hit
GGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAGATCCCA2130.11123412433155079No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA2130.11123412433155079No Hit
CTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTAC2070.10810076871657753No Hit
CTTTACGGGGCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGA2070.10810076871657753No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC2040.1065340909090909No Hit
GGCCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC2040.1065340909090909No Hit
ATCTGACCCTTTACCCCGTTGCCCGGCAACGGCCAGGCCTGTGCCAAGTG2030.10601186497326202No Hit
CAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCC2000.1044451871657754No Hit
CAGGTAGTTTTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGT1980.10340073529411765No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG1980.10340073529411765No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT1980.10340073529411765No Hit
ACTTTACGGGGCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGG1980.10340073529411765No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT1970.10287850935828877No Hit
ATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGC1970.10287850935828877No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTTT1950.101834057486631No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGT1950.101834057486631No Hit
AATAATTGCTTGCCTGAGTGCAGTATGGTGAGGTGAACAATGTTCAGGAG1950.101834057486631No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG1940.10131183155080213No Hit
CAAGGCAGGTCCTCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGATC1940.10131183155080213No Hit
CAGTCAATGAGAAACGAAGACTACAATTAATTATGCCTGCTAGGTTTTAT1920.1002673796791444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)