FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191488
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA44462.321816510695187No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTCCTAAT18710.977084725935829No Hit
CCTGCCTTGTCGTCTAATAACAGTAGTCTCCGGAAGTGTTGATAAGATAG17270.9018841911764706No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT17040.8898729946524063No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13400.6997827540106952No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13080.6830715240641712No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12640.6600935828877006No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT10410.5436371991978609No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC10240.53475935828877No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCATTTCCTAATATACATTTACA9720.5076036096256685No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9100.47522560160427807No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTC8990.46948111631016043No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8210.42874749331550804No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7990.4172585227272727No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT7930.4141251671122994No Hit
ACAATACAGGTCCTCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT7780.40629177807486627No Hit
CTTTAACCCTCATTGGAAAACACCTTCATTTCCTAATATACATTTACACC7420.3874916443850267No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6680.34884692513368987No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6620.34571356951871657No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA6440.3363135026737968No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6320.3300467914438503No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6300.32900233957219255No Hit
GATCCAGCATCTAGGGACCTAGTAGTGAGTTATGTCAACACTAATATGGG6160.3216911764705882No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6050.3159466911764706No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTCCTAATATACA5580.29140207219251335No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTTAT5480.2861798128342246No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTCCT5420.28304645721925137No Hit
CCTGTATTGTCGTCTAATAACAGTAGTCTCCGGAAGTGTTGATAAGATAG5100.26633522727272724No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5090.2658130013368984No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCTCCGTG5050.2637240975935829No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5050.2637240975935829No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4890.25536848262032086No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACCACCGTG4560.2381350267379679No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA4500.23500167112299464No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA4480.2339572192513369No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA4440.2318683155080214No Hit
GCATAGCCTGAGGATGAGTTTGTCTCAAAGGTGGAGACAGCGGGGTAGGC4350.2271682820855615No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4340.22664605614973263No Hit
ACTACACACCGTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGT4270.22299047459893045No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA4240.22142379679144386No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4230.220901570855615No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4200.21933489304812837No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4170.21776821524064172No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4040.21097927807486633No Hit
GAATATGGTGACCCACAAAATGAGGCGCTACGTGTTGTTTCTCTCTTATA3980.20784592245989306No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG3850.20105698529411764No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT3630.18956801470588236No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC3620.18904578877005349No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG3560.18591243315508021No Hit
GTCAATGAGAAACGAAGACTACAATTAATTATGCCTGCTAGGTTTTATCC3530.1843457553475936No Hit
GGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTT3410.17807904411764705No Hit
TCCTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTG3320.17337901069518716No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA3300.1723345588235294No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3250.16972342914438504No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG3240.16920120320855617No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCGTTTCTCA3210.16763452540106952No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA3200.16711229946524064No Hit
GTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATG3130.1634567179144385No Hit
CTTTATGCCCATATTAGTGTTGACATAACTCACTACTAGGTCCCTAGATG3120.16293449197860962No Hit
CTCACAGTCAATGAGAAACGAAGACTACAATTAATTATGCCTGCTAGGTT3070.16032336229946523No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3040.1587566844919786No Hit
CCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACT2940.15353442513368984No Hit
ACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTTCCTAATAT2920.1524899732620321No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2870.1498788435828877No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG2870.1498788435828877No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT2810.14674548796791445No Hit
TTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCAT2780.1451788101604278No Hit
CATTGGAAAACACCTTCATTTCCTAATATACATTTACACCAAGACATTAT2760.14413435828877005No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTAT2740.1430899064171123No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG2710.14152322860962568No Hit
AGAATATGGTGACCCACAAAATGAGGCGCTACGTGTTGTTTCTCTCTTAT2670.13943432486631016No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG2670.13943432486631016No Hit
GTGTAAATGTATATTAGGAAATGAAGGTGTTTTCCAATGAGGGTTAAAGA2670.13943432486631016No Hit
ATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAA2610.1363009692513369No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG2590.13525651737967914No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT2560.1336898395721925No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2500.13055648395721925No Hit
ATCTGACCCTTTACCCCGTTGCCCGGCAACGGCCAGGCCTGTGCCAAGTG2470.12898980614973263No Hit
GCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAAC2410.12585645053475936No Hit
CCTTCATTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGTGA2410.12585645053475936No Hit
GTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATACC2410.12585645053475936No Hit
ATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC2370.12376754679144385No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC2370.12376754679144385No Hit
AGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAGATCCC2350.12272309491978609No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2340.12220086898395721No Hit
GTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCTCCGTGCG2320.12115641711229948No Hit
GTTAACAGGTAGTTTTCTAAAACAGTCTTTGATTTTCTGTATGATGTGAT2300.12011196524064172No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG2280.11906751336898395No Hit
GGAAAACACCTTCATTTCCTAATATACATTTACACCAAGACATTATCAAA2280.11906751336898395No Hit
CTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTAC2280.11906751336898395No Hit
TTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCT2270.11854528743315508No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC2240.11697860962566844No Hit
CAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTT2240.11697860962566844No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG2210.11541193181818182No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC2200.11488970588235295No Hit
ACGTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA2190.11436747994652406No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTTT2190.11436747994652406No Hit
AACTAGATGTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAAT2190.11436747994652406No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2170.11332302807486631No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA2170.11332302807486631No Hit
ATACTACACACCGTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCA2120.11071189839572193No Hit
CTACAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTT2120.11071189839572193No Hit
GGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCGTTTCTCATTGAC2110.11018967245989306No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCT2090.1091452205882353No Hit
GGCTATAGCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA2080.10862299465240642No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA2080.10862299465240642No Hit
ATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCATTT2070.10810076871657753No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC2060.10757854278074866No Hit
GGCCAGGCCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC2050.10705631684491979No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA2040.1065340909090909No Hit
ATCCAATGCTATAGCCCATAAAATTTAAAGAGTAACCCCATCTTTTAGTT2000.1044451871657754No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA2000.1044451871657754No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT2000.1044451871657754No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTACGGGAGAGAACAACAGAGT1990.10392296122994653No Hit
GGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAGATCCCA1970.10287850935828877No Hit
ATACTGCACTCAGGCAAGCAATTATTTGCTGGGGGGAACTAATGACTCTA1970.10287850935828877No Hit
CAATACAGGTCCTCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGATC1970.10287850935828877No Hit
CCTCTATGTATCCCTCCTGTTGTTGTACCAAACCTACGGACGGAAATTGC1960.10235628342245989No Hit
TTGGAAAACACCTTCATTTCCTAATATACATTTACACCAAGACATTATCA1950.101834057486631No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC1940.10131183155080213No Hit
ACCTAGTAGTGAGTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCAA1930.10078960561497326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)