FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462563
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG28530.616780849311337No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG21070.4555055203291227No Hit
ATCCTAAACTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCACAT19930.430860228768838No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT19890.4299954816965473No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15830.3422236538590419No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAA15270.3301171948469722No Hit
GTCTAGACTCGTGGTTGACTTCTCTCAATTTTCTAGGGGGAGTACCCACG14150.3059042768228328No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13270.28687984123243754No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13200.2853665338559288No Hit
GTTCAGGATAATGTGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA12510.2704496468589143No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12000.25942412168720796No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11870.2566136937022633No Hit
GTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAA11740.25380326571731854No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11730.2535870789492458No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11710.2531547054131005No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTCAT11570.25012809066008307No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTGTGAATAATGTCCTCCTGT11390.24623672883477493No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10970.23715688457572265No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT10940.23650832427150464No Hit
GTCTATTGACAGGCAATTTTCGAAAACATTGTTTGATTTTTTGTACAATA10130.21899719605761808No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCT9910.2142410871600193No Hit
GCTTAGAGTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACAT9870.2133763400877286No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCGCCATA9820.21229540624736523No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9800.21186303271121987No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9510.20559361643711235No Hit
TTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAATTTTCGAAAAC9310.20126988107565888No Hit
CAATAGGTCTATTGACAGGCAATTTTCGAAAACATTGTTTGATTTTTTGT9110.19694614571420543No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGAAAATTGCCTGTCA9080.19629758540998737No Hit
GGTCTATTGACAGGCAATTTTCGAAAACATTGTTTGATTTTTTGTACAAT8950.19348715742504263No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8840.19110910297624323No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8810.19046054267202522No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8510.183974939629845No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8500.18375875286177235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8320.1798673910364642No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT8300.17943501750031887No Hit
GAGTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGT7800.16862567909668522No Hit
GGATAATGTGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGTTT7800.16862567909668522No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7510.16235626282257767No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7510.16235626282257767No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATGTGGTTTAATGCC7380.15954583483763293No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7330.15846490099726956No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7320.1582487142291969No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCGCCATATT7310.15803252746112423No Hit
TAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGATAG7270.15716778038883353No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7230.15630303331654283No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7220.15608684654847016No Hit
ACATTATTCACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAA6880.14873649643399925No Hit
CTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTCATATTCATTTACAGG6850.14808793612978124No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6830.1476555625936359No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAAACTTACCAAATAT6700.14484513460869114No Hit
GTTCTATCCTAAACTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6640.1435480140002551No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCACAAGACCGCCGTG6630.1433318272321824No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6520.14095377278338303No Hit
CATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6490.140305212479165No Hit
CATCCACTACCAGCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAA6410.13857571833458363No Hit
CTCATAAGGTGGGAAACTTTACTGGCCTCTATTCTTCTACTGTACCTGTC6410.13857571833458363No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAC6370.13771097126229292No Hit
GTATCCAGTTGGCAGCACACCCTAGCAGCCATGGAAAGGAGGTGTATTTC6330.13684622419000222No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACAAAAA6210.13425198297313012No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6110.13209011529240341No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC6100.13187392852433075No Hit
CTTTTACACAATGTGGCTATCCTGCTTTAATGCCTTTATATGCATGTATA6100.13187392852433075No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6070.13122536822011271No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTT5960.1288473137713133No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGCCTCTATTCTTCTACTG5930.1281987534670953No Hit
CATTTACAGGAGGACATTATTCACAGATGTCAACAATTTGTGGGCCCTCT5920.1279825666990226No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5900.12755019316287727No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5890.1273340063948046No Hit
ACTGGATACTGCGCGGGACGTCCTTTGTCTACGTCCCGTCGGCGCTGAAT5880.1271178196267319No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGCCTCTATTC5800.12538832548215054No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5770.1247397651779325No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5710.12344264456949647No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5660.12236171072913311No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACGTTTCC5640.12192933719298778No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5640.12192933719298778No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC5620.12149696365684243No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5610.12128077688876975No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5510.11911890920804301No Hit
GTCAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT5510.11911890920804301No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATT5490.11868653567189767No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG5460.11803797536767964No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACA5420.11717322829538895No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5380.11630848122309825No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTA5330.1152275473827349No Hit
CCACAAAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCAT5320.11501136061466222No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5320.11501136061466222No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTCCCTGACTGCCGATTG5310.11479517384658955No Hit
TAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATGTAA5280.11414661354237153No Hit
CAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5280.11414661354237153No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5270.11393042677429885No Hit
ATTCACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAG5250.1134980532381535No Hit
GATTAACTGCATGTTCAGGATAATGTGGTTTAATGCCTTTGTCCAAGGGC5200.11241711939779014No Hit
GCTAGGTTCTATCCTAAACTTACCAAATATTTGCCCTTGGACAAAGGCAT5150.11133618555742676No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5130.11090381202128143No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5030.10874194434055469No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5000.10809338403633667No Hit
ATTTACAGGAGGACATTATTCACAGATGTCAACAATTTGTGGGCCCTCTT4960.10722863696404597No Hit
TGCTTAGAGTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACA4930.10658007665982797No Hit
CTCCAAAAGACACCAAGTACTCAAGAACAGTATCTCTTCCAAACGTAAGA4860.10506676928331925No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCT4850.10485058251524658No Hit
TACATATCCCATGAAGTTAAGGGAATAGCCCCAACGTTTGGTTTTATTAG4810.10398583544295587No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4790.10355346190681053No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC4780.10333727513873786No Hit
CCTCTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT4710.10182396776222914No Hit
AGTTAAGGGAATAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4660.10074303392186579No Hit
TCACCATACAGCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGA4630.10009447361764776No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4630.10009447361764776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)